Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius

Sea urchin (Strongylocentrotus intermedius) is an economically important mariculture species in Asia, and its gonads are the only edible part. The efficiency of genetic breeding in sea urchins is hampered due to the inability to distinguish gender by appearance. In this study, we first identified a...

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Autores principales:	Ya-Lun Han, Zhi-Hui Sun, Shuai Chang, Bin Wen, Jian Song, Ran-Tao Zuo, Ya-Qing Chang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SNP sea urchin estradiol 2b-RAD sex identification Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/0858192400424e8da5093256bf9421cf
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spelling	oai:doaj.org-article:0858192400424e8da5093256bf9421cf2021-11-11T09:41:29ZApplication of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius1664-239210.3389/fendo.2021.756530https://doaj.org/article/0858192400424e8da5093256bf9421cf2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fendo.2021.756530/fullhttps://doaj.org/toc/1664-2392Sea urchin (Strongylocentrotus intermedius) is an economically important mariculture species in Asia, and its gonads are the only edible part. The efficiency of genetic breeding in sea urchins is hampered due to the inability to distinguish gender by appearance. In this study, we first identified a sex-associated single nucleotide polymorphism (SNP) by combining type IIB endonuclease restriction site-associated DNA sequencing (2b-RAD-seq) and genome survey. Importantly, this SNP is located within spata4, a gene specifically expressed in male. Knocking down of spata4 by RNA interference (RNAi) in male individuals led to the downregulation of other conserved testis differentiation-related genes and germ cell marker genes. We also revealed that sex ratio in this validated culture population of S. intermedius is not 1:1. Moreover, after a 58-day feeding experiment with estradiol, the expression levels of several conserved genes that are related to testis differentiation, ovary differentiation, and estrogen metabolism were dynamically changed. Taken together, our results will contribute toward improving breeding efficiency, developing sex-controlled breeding, and providing a solid base for understanding sex determination mechanisms in sea urchins.Ya-Lun HanZhi-Hui SunShuai ChangBin WenJian SongRan-Tao ZuoYa-Qing ChangFrontiers Media S.A.articleSNPsea urchinestradiol2b-RADsex identificationDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENFrontiers in Endocrinology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SNP sea urchin estradiol 2b-RAD sex identification Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	SNP sea urchin estradiol 2b-RAD sex identification Diseases of the endocrine glands. Clinical endocrinology RC648-665 Ya-Lun Han Zhi-Hui Sun Shuai Chang Bin Wen Jian Song Ran-Tao Zuo Ya-Qing Chang Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
description	Sea urchin (Strongylocentrotus intermedius) is an economically important mariculture species in Asia, and its gonads are the only edible part. The efficiency of genetic breeding in sea urchins is hampered due to the inability to distinguish gender by appearance. In this study, we first identified a sex-associated single nucleotide polymorphism (SNP) by combining type IIB endonuclease restriction site-associated DNA sequencing (2b-RAD-seq) and genome survey. Importantly, this SNP is located within spata4, a gene specifically expressed in male. Knocking down of spata4 by RNA interference (RNAi) in male individuals led to the downregulation of other conserved testis differentiation-related genes and germ cell marker genes. We also revealed that sex ratio in this validated culture population of S. intermedius is not 1:1. Moreover, after a 58-day feeding experiment with estradiol, the expression levels of several conserved genes that are related to testis differentiation, ovary differentiation, and estrogen metabolism were dynamically changed. Taken together, our results will contribute toward improving breeding efficiency, developing sex-controlled breeding, and providing a solid base for understanding sex determination mechanisms in sea urchins.
format	article
author	Ya-Lun Han Zhi-Hui Sun Shuai Chang Bin Wen Jian Song Ran-Tao Zuo Ya-Qing Chang
author_facet	Ya-Lun Han Zhi-Hui Sun Shuai Chang Bin Wen Jian Song Ran-Tao Zuo Ya-Qing Chang
author_sort	Ya-Lun Han
title	Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
title_short	Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
title_full	Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
title_fullStr	Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
title_full_unstemmed	Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius
title_sort	application of snp in genetic sex identification and effect of estradiol on gene expression of sex-related genes in strongylocentrotus intermedius
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0858192400424e8da5093256bf9421cf
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Application of SNP in Genetic Sex Identification and Effect of Estradiol on Gene Expression of Sex-Related Genes in Strongylocentrotus intermedius

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