Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides

Susceptible host plants challenged by fungal pathogens can display different types of lesions, which can be attributed to environmental factors affecting the nature of interactions between the host and pathogen. During our survey of apple anthracnose in Korea, two distinct types of disease symptoms,...

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Autores principales:	Wonsu Cheon, Young Soo Kim, Kotnala Balaraju, Younmi Lee, Hyeok Tae Kwon, Junhyun Jeon, Yongho Jeon
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cellophane membrane Colletotrichum gloeosporioides glyoxylate cycle pathogenicity progressive symptoms qRT-PCR Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/085920d8ae2d4dc4a5186dd2904e54f2
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spelling	oai:doaj.org-article:085920d8ae2d4dc4a5186dd2904e54f22021-11-08T14:07:21ZDistinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides1664-302X10.3389/fmicb.2021.743776https://doaj.org/article/085920d8ae2d4dc4a5186dd2904e54f22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.743776/fullhttps://doaj.org/toc/1664-302XSusceptible host plants challenged by fungal pathogens can display different types of lesions, which can be attributed to environmental factors affecting the nature of interactions between the host and pathogen. During our survey of apple anthracnose in Korea, two distinct types of disease symptoms, designated as progressive (PS) and static symptoms (SS), were recognized. PS is a typical, rapidly enlarging symptom of apple anthracnose, while SS is a small, dark speck that does not expand further until the harvesting season. Isolation and genotyping of pathogens from disease lesions suggested that all of them belong to Colletotrichum gloeosporioides, a well-known causal agent of apple anthracnose. Two types of isolates were comparable in growth on media, spore germination and appressorium formation, virulence test on fruits at various temperature conditions. Furthermore, they were analyzed at the molecular level by a phylogenetic tree, RNA-seq, and expression of virulence gene. However, the SS isolates were defective in appressorium-mediated penetration into the underlying substratum. RNA-seq analysis of PS and SS isolates showed that distinct transcriptional programs underlie the development of different types of anthracnose symptoms in host plants. One downregulated gene in SS encoded isocitrate lyase is essential for disease development via its involvement in the glyoxylate cycle. It partly explains why SS is less virulent than PS on host plants. Overall, our work challenges the traditional view on the development of different lesion types and provides valuable insights into variations that exist in the pathogen population.Wonsu CheonWonsu CheonYoung Soo KimKotnala BalarajuYounmi LeeYounmi LeeHyeok Tae KwonJunhyun JeonYongho JeonFrontiers Media S.A.articlecellophane membraneColletotrichum gloeosporioidesglyoxylate cyclepathogenicityprogressive symptomsqRT-PCRMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellophane membrane Colletotrichum gloeosporioides glyoxylate cycle pathogenicity progressive symptoms qRT-PCR Microbiology QR1-502
spellingShingle	cellophane membrane Colletotrichum gloeosporioides glyoxylate cycle pathogenicity progressive symptoms qRT-PCR Microbiology QR1-502 Wonsu Cheon Wonsu Cheon Young Soo Kim Kotnala Balaraju Younmi Lee Younmi Lee Hyeok Tae Kwon Junhyun Jeon Yongho Jeon Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
description	Susceptible host plants challenged by fungal pathogens can display different types of lesions, which can be attributed to environmental factors affecting the nature of interactions between the host and pathogen. During our survey of apple anthracnose in Korea, two distinct types of disease symptoms, designated as progressive (PS) and static symptoms (SS), were recognized. PS is a typical, rapidly enlarging symptom of apple anthracnose, while SS is a small, dark speck that does not expand further until the harvesting season. Isolation and genotyping of pathogens from disease lesions suggested that all of them belong to Colletotrichum gloeosporioides, a well-known causal agent of apple anthracnose. Two types of isolates were comparable in growth on media, spore germination and appressorium formation, virulence test on fruits at various temperature conditions. Furthermore, they were analyzed at the molecular level by a phylogenetic tree, RNA-seq, and expression of virulence gene. However, the SS isolates were defective in appressorium-mediated penetration into the underlying substratum. RNA-seq analysis of PS and SS isolates showed that distinct transcriptional programs underlie the development of different types of anthracnose symptoms in host plants. One downregulated gene in SS encoded isocitrate lyase is essential for disease development via its involvement in the glyoxylate cycle. It partly explains why SS is less virulent than PS on host plants. Overall, our work challenges the traditional view on the development of different lesion types and provides valuable insights into variations that exist in the pathogen population.
format	article
author	Wonsu Cheon Wonsu Cheon Young Soo Kim Kotnala Balaraju Younmi Lee Younmi Lee Hyeok Tae Kwon Junhyun Jeon Yongho Jeon
author_facet	Wonsu Cheon Wonsu Cheon Young Soo Kim Kotnala Balaraju Younmi Lee Younmi Lee Hyeok Tae Kwon Junhyun Jeon Yongho Jeon
author_sort	Wonsu Cheon
title	Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
title_short	Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
title_full	Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
title_fullStr	Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
title_full_unstemmed	Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides
title_sort	distinct transcriptional programs underlie differences in virulence of isolates on host plants in a fungal pathogen, colletotrichum gloeosporioides
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/085920d8ae2d4dc4a5186dd2904e54f2
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Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides

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