Genomic characterization of the Taylorella genus.

The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the rec...

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Autores principales:	Laurent Hébert, Bouziane Moumen, Nicolas Pons, Fabien Duquesne, Marie-France Breuil, Didier Goux, Jean-Michel Batto, Claire Laugier, Pierre Renault, Sandrine Petry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/085b17e6fb684b7f8526fc80ddb9c0f8
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spelling	oai:doaj.org-article:085b17e6fb684b7f8526fc80ddb9c0f82021-11-18T07:31:02ZGenomic characterization of the Taylorella genus.1932-620310.1371/journal.pone.0029953https://doaj.org/article/085b17e6fb684b7f8526fc80ddb9c0f82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22235352/?tool=EBIhttps://doaj.org/toc/1932-6203The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttinin-related proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, paving the way for future functional investigations into this largely unknown genus.Laurent HébertBouziane MoumenNicolas PonsFabien DuquesneMarie-France BreuilDidier GouxJean-Michel BattoClaire LaugierPierre RenaultSandrine PetryPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29953 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laurent Hébert Bouziane Moumen Nicolas Pons Fabien Duquesne Marie-France Breuil Didier Goux Jean-Michel Batto Claire Laugier Pierre Renault Sandrine Petry Genomic characterization of the Taylorella genus.
description	The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3% GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttinin-related proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, paving the way for future functional investigations into this largely unknown genus.
format	article
author	Laurent Hébert Bouziane Moumen Nicolas Pons Fabien Duquesne Marie-France Breuil Didier Goux Jean-Michel Batto Claire Laugier Pierre Renault Sandrine Petry
author_facet	Laurent Hébert Bouziane Moumen Nicolas Pons Fabien Duquesne Marie-France Breuil Didier Goux Jean-Michel Batto Claire Laugier Pierre Renault Sandrine Petry
author_sort	Laurent Hébert
title	Genomic characterization of the Taylorella genus.
title_short	Genomic characterization of the Taylorella genus.
title_full	Genomic characterization of the Taylorella genus.
title_fullStr	Genomic characterization of the Taylorella genus.
title_full_unstemmed	Genomic characterization of the Taylorella genus.
title_sort	genomic characterization of the taylorella genus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/085b17e6fb684b7f8526fc80ddb9c0f8
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Genomic characterization of the Taylorella genus.

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