Development of dual‐inducible duet‐expression vectors for tunable gene expression control and CRISPR interference‐based gene repression in Pseudomonas putida KT2440
Summary The development of P. putida as an industrial host requires a sophisticated molecular toolbox for strain improvement, including vectors for gene expression and repression. To augment existing expression plasmids for metabolic engineering, we developed a series of dual‐inducible duet‐expressi...
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Main Authors: | Rahul Gauttam, Aindrila Mukhopadhyay, Blake A. Simmons, Steven W. Singer |
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Format: | article |
Language: | EN |
Published: |
Wiley
2021
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Subjects: | |
Online Access: | https://doaj.org/article/085bd22aa4b04e3fa97dece028780c1a |
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