Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit

Abstract Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has a limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of fruit ripening, and members o...

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Autores principales:	C. Carrasco-Orellana, Y. Stappung, A. Mendez-Yañez, A. C. Allan, R. V. Espley, B. J. Plunkett, M. A. Moya-Leon, R. Herrera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/085fc4f76c9d48e3ae216661b1bb9c3d
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spelling	oai:doaj.org-article:085fc4f76c9d48e3ae216661b1bb9c3d2021-12-02T15:08:39ZCharacterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit10.1038/s41598-018-28226-y2045-2322https://doaj.org/article/085fc4f76c9d48e3ae216661b1bb9c3d2018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28226-yhttps://doaj.org/toc/2045-2322Abstract Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has a limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of fruit ripening, and members of the NAC family have been implicated in cell wall remodeling. FcNAC1 was isolated from F. chiloensis fruit, coding a protein of 332 amino acid residues and displaying a characteristic NAC domain at the N terminus. FcNAC1 protein showed nuclear localization. An increase in transcript level was observed during ripening. A sequence of 1488 bp of FcNAC1 promoter was obtained. In silico analysis identified cis elements able to respond to some hormones and Secondary wall NAC binding elements (SNBE), and responding to auxin and ABA. A structural model of FcNAC1 provided evidence for interaction with DNA sequences containing SNBE, while a dual luciferase assay confirmed the transcriptional activation by FcNAC1 of the promoter of FcPL, a gene involved in cell wall remodeling in F. chiloensis fruit. The results suggest the participation of FcNAC1 during ripening development of strawberry fruit, by regulating pectin metabolism during softening.C. Carrasco-OrellanaY. StappungA. Mendez-YañezA. C. AllanR. V. EspleyB. J. PlunkettM. A. Moya-LeonR. HerreraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. Carrasco-Orellana Y. Stappung A. Mendez-Yañez A. C. Allan R. V. Espley B. J. Plunkett M. A. Moya-Leon R. Herrera Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
description	Abstract Fragaria chiloensis is a strawberry endemic from Chile with attractive white-pink fruit, pleasant aroma and taste. However, this fruit has a limited post-harvest period due to fast softening. Several transcription factors (TFs) are involved in the regulation of fruit ripening, and members of the NAC family have been implicated in cell wall remodeling. FcNAC1 was isolated from F. chiloensis fruit, coding a protein of 332 amino acid residues and displaying a characteristic NAC domain at the N terminus. FcNAC1 protein showed nuclear localization. An increase in transcript level was observed during ripening. A sequence of 1488 bp of FcNAC1 promoter was obtained. In silico analysis identified cis elements able to respond to some hormones and Secondary wall NAC binding elements (SNBE), and responding to auxin and ABA. A structural model of FcNAC1 provided evidence for interaction with DNA sequences containing SNBE, while a dual luciferase assay confirmed the transcriptional activation by FcNAC1 of the promoter of FcPL, a gene involved in cell wall remodeling in F. chiloensis fruit. The results suggest the participation of FcNAC1 during ripening development of strawberry fruit, by regulating pectin metabolism during softening.
format	article
author	C. Carrasco-Orellana Y. Stappung A. Mendez-Yañez A. C. Allan R. V. Espley B. J. Plunkett M. A. Moya-Leon R. Herrera
author_facet	C. Carrasco-Orellana Y. Stappung A. Mendez-Yañez A. C. Allan R. V. Espley B. J. Plunkett M. A. Moya-Leon R. Herrera
author_sort	C. Carrasco-Orellana
title	Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
title_short	Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
title_full	Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
title_fullStr	Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
title_full_unstemmed	Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit
title_sort	characterization of a ripening-related transcription factor fcnac1 from fragaria chiloensis fruit
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/085fc4f76c9d48e3ae216661b1bb9c3d
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Characterization of a ripening-related transcription factor FcNAC1 from Fragaria chiloensis fruit

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