Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy

Abstract The ovalbumin-induced (OVA) chronic allergic airways murine model is a well-established model for investigating pre-clinical therapies for chronic allergic airways diseases, such as asthma. Here, we examined the effects of several experimental compounds with potential anti-asthmatic effects...

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Autores principales:	Nadia Mazarakis, Jitraporn Vongsvivut, Keith R. Bambery, Katherine Ververis, Mark J. Tobin, Simon G. Royce, Chrishan S. Samuel, Kenneth J. Snibson, Paul V. Licciardi, Tom C. Karagiannis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0864f305e454477a82fdd3b364b6f67a
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spelling	oai:doaj.org-article:0864f305e454477a82fdd3b364b6f67a2021-12-02T15:33:10ZInvestigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy10.1038/s41598-020-68671-22045-2322https://doaj.org/article/0864f305e454477a82fdd3b364b6f67a2020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68671-2https://doaj.org/toc/2045-2322Abstract The ovalbumin-induced (OVA) chronic allergic airways murine model is a well-established model for investigating pre-clinical therapies for chronic allergic airways diseases, such as asthma. Here, we examined the effects of several experimental compounds with potential anti-asthmatic effects including resveratrol (RV), relaxin (RLN), l-sulforaphane (LSF), valproic acid (VPA), and trichostatin A (TSA) using both a prevention and reversal model of chronic allergic airways disease. We undertook a novel analytical approach using focal plane array (FPA) and synchrotron Fourier-transform infrared (S-FTIR) microspectroscopic techniques to provide new insights into the mechanisms of action of these experimental compounds. Apart from the typical biological effects, S-FTIR microspectroscopy was able to detect changes in nucleic acids and protein acetylation. Further, we validated the reduction in collagen deposition induced by each experimental compound evaluated. Although this has previously been observed with conventional histological methods, the S-FTIR technique has the advantage of allowing identification of the type of collagen present. More generally, our findings highlight the potential utility of S-FTIR and FPA-FTIR imaging techniques in enabling a better mechanistic understanding of novel asthma therapeutics.Nadia MazarakisJitraporn VongsvivutKeith R. BamberyKatherine VerverisMark J. TobinSimon G. RoyceChrishan S. SamuelKenneth J. SnibsonPaul V. LicciardiTom C. KaragiannisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadia Mazarakis Jitraporn Vongsvivut Keith R. Bambery Katherine Ververis Mark J. Tobin Simon G. Royce Chrishan S. Samuel Kenneth J. Snibson Paul V. Licciardi Tom C. Karagiannis Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
description	Abstract The ovalbumin-induced (OVA) chronic allergic airways murine model is a well-established model for investigating pre-clinical therapies for chronic allergic airways diseases, such as asthma. Here, we examined the effects of several experimental compounds with potential anti-asthmatic effects including resveratrol (RV), relaxin (RLN), l-sulforaphane (LSF), valproic acid (VPA), and trichostatin A (TSA) using both a prevention and reversal model of chronic allergic airways disease. We undertook a novel analytical approach using focal plane array (FPA) and synchrotron Fourier-transform infrared (S-FTIR) microspectroscopic techniques to provide new insights into the mechanisms of action of these experimental compounds. Apart from the typical biological effects, S-FTIR microspectroscopy was able to detect changes in nucleic acids and protein acetylation. Further, we validated the reduction in collagen deposition induced by each experimental compound evaluated. Although this has previously been observed with conventional histological methods, the S-FTIR technique has the advantage of allowing identification of the type of collagen present. More generally, our findings highlight the potential utility of S-FTIR and FPA-FTIR imaging techniques in enabling a better mechanistic understanding of novel asthma therapeutics.
format	article
author	Nadia Mazarakis Jitraporn Vongsvivut Keith R. Bambery Katherine Ververis Mark J. Tobin Simon G. Royce Chrishan S. Samuel Kenneth J. Snibson Paul V. Licciardi Tom C. Karagiannis
author_facet	Nadia Mazarakis Jitraporn Vongsvivut Keith R. Bambery Katherine Ververis Mark J. Tobin Simon G. Royce Chrishan S. Samuel Kenneth J. Snibson Paul V. Licciardi Tom C. Karagiannis
author_sort	Nadia Mazarakis
title	Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
title_short	Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
title_full	Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
title_fullStr	Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
title_full_unstemmed	Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
title_sort	investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron fourier-transform infrared microspectroscopy
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0864f305e454477a82fdd3b364b6f67a
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Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy

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