Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
The emergence of carbapenem-resistant <i>Proteus</i> represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a <i>Proteus</i> isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carb...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/08675537690a40faadf35533a1ae944a |
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| Sumario: | The emergence of carbapenem-resistant <i>Proteus</i> represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a <i>Proteus</i> isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carbapenems. Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>. Sequence analysis of pPvSC3-like plasmids showed that they share a conserved backbone but have a diverse accessory module with complex chimera structures bearing abundant resistance genes, which are facilitated by transposons and/or homologous recombination. The acquisition of <i>bla</i><sub>NDM-1</sub> in pNDM_NMG38-2 was due to the IS<i>CR1</i>-mediated integration event. Comprehensive analysis of the <i>lnu(G)-</i>bearing cassettes carried by bacterial plasmids or chromosomes revealed a diversification of its genetic contexts, with Tn<i>6260</i> and IS<i>Pst2</i> elements being the leading contributors to the dissemination of <i>lnu(G)</i> in <i>Enterococcus</i> and Enterobacteriaceae, respectively. In conclusion, this study provides a better understanding of the genetic features of pPvSC3-like plasmids, which represent a novel plasmid group as a vehicle mediating the dissemination of <i>bla</i><sub>NDM-1</sub> among bacteria species. Moreover, our results highlight the central roles of Tn<i>6260</i> and IS<i>Pst2</i> in the spread of <i>lnu(G)</i>. |
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