Genomic Characterization of a Proteus sp. Strain of Animal Origin Co-Carrying blaNDM-1 and lnu(G)

The emergence of carbapenem-resistant Proteus represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a Proteus isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carb...

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Autores principales:	Ying Li, Yichuan Qiu, Junping She, Xu Wang, Xiaoyi Dai, Luhua Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carbapenem IS<i>CR1</i> pPvSC3-like IS<i>Pst2</i> Tn<i>6260</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/08675537690a40faadf35533a1ae944a
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spelling	oai:doaj.org-article:08675537690a40faadf35533a1ae944a2021-11-25T16:24:58ZGenomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>10.3390/antibiotics101114112079-6382https://doaj.org/article/08675537690a40faadf35533a1ae944a2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1411https://doaj.org/toc/2079-6382The emergence of carbapenem-resistant <i>Proteus</i> represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a <i>Proteus</i> isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carbapenems. Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>. Sequence analysis of pPvSC3-like plasmids showed that they share a conserved backbone but have a diverse accessory module with complex chimera structures bearing abundant resistance genes, which are facilitated by transposons and/or homologous recombination. The acquisition of <i>bla</i><sub>NDM-1</sub> in pNDM_NMG38-2 was due to the IS<i>CR1</i>-mediated integration event. Comprehensive analysis of the <i>lnu(G)-</i>bearing cassettes carried by bacterial plasmids or chromosomes revealed a diversification of its genetic contexts, with Tn<i>6260</i> and IS<i>Pst2</i> elements being the leading contributors to the dissemination of <i>lnu(G)</i> in <i>Enterococcus</i> and Enterobacteriaceae, respectively. In conclusion, this study provides a better understanding of the genetic features of pPvSC3-like plasmids, which represent a novel plasmid group as a vehicle mediating the dissemination of <i>bla</i><sub>NDM-1</sub> among bacteria species. Moreover, our results highlight the central roles of Tn<i>6260</i> and IS<i>Pst2</i> in the spread of <i>lnu(G)</i>.Ying LiYichuan QiuJunping SheXu WangXiaoyi DaiLuhua ZhangMDPI AGarticlecarbapenemIS<i>CR1</i>pPvSC3-likeIS<i>Pst2</i>Tn<i>6260</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1411, p 1411 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbapenem IS<i>CR1</i> pPvSC3-like IS<i>Pst2</i> Tn<i>6260</i> Therapeutics. Pharmacology RM1-950
spellingShingle	carbapenem IS<i>CR1</i> pPvSC3-like IS<i>Pst2</i> Tn<i>6260</i> Therapeutics. Pharmacology RM1-950 Ying Li Yichuan Qiu Junping She Xu Wang Xiaoyi Dai Luhua Zhang Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
description	The emergence of carbapenem-resistant <i>Proteus</i> represents a serious threat to global public health due to limited antibiotic treatment options. Here, we characterize a <i>Proteus</i> isolate NMG38-2 of swine origin that exhibits extensive drug resistance, including carbapenems. Whole-genome sequencing based on Illumina and MinION platforms showed that NMG38-2 contains 24 acquired antibiotic resistance genes and three plasmids, among which, pNDM_NMG38-2, a pPvSC3-like plasmid, is transferable and co-carries <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>. Sequence analysis of pPvSC3-like plasmids showed that they share a conserved backbone but have a diverse accessory module with complex chimera structures bearing abundant resistance genes, which are facilitated by transposons and/or homologous recombination. The acquisition of <i>bla</i><sub>NDM-1</sub> in pNDM_NMG38-2 was due to the IS<i>CR1</i>-mediated integration event. Comprehensive analysis of the <i>lnu(G)-</i>bearing cassettes carried by bacterial plasmids or chromosomes revealed a diversification of its genetic contexts, with Tn<i>6260</i> and IS<i>Pst2</i> elements being the leading contributors to the dissemination of <i>lnu(G)</i> in <i>Enterococcus</i> and Enterobacteriaceae, respectively. In conclusion, this study provides a better understanding of the genetic features of pPvSC3-like plasmids, which represent a novel plasmid group as a vehicle mediating the dissemination of <i>bla</i><sub>NDM-1</sub> among bacteria species. Moreover, our results highlight the central roles of Tn<i>6260</i> and IS<i>Pst2</i> in the spread of <i>lnu(G)</i>.
format	article
author	Ying Li Yichuan Qiu Junping She Xu Wang Xiaoyi Dai Luhua Zhang
author_facet	Ying Li Yichuan Qiu Junping She Xu Wang Xiaoyi Dai Luhua Zhang
author_sort	Ying Li
title	Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
title_short	Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
title_full	Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
title_fullStr	Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
title_full_unstemmed	Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>
title_sort	genomic characterization of a <i>proteus</i> sp. strain of animal origin co-carrying <i>bla</i><sub>ndm-1</sub> and <i>lnu(g)</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/08675537690a40faadf35533a1ae944a
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Genomic Characterization of a <i>Proteus</i> sp. Strain of Animal Origin Co-Carrying <i>bla</i><sub>NDM-1</sub> and <i>lnu(G)</i>

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