Reference genes for gene expression studies in the mouse heart

Abstract To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of pr...

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Autores principales:	Adrián Ruiz-Villalba, Andrea Mattiotti, Quinn D. Gunst, Sara Cano-Ballesteros, Maurice J. B. van den Hoff, Jan M. Ruijter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/087cf85ea5924b77a3edc2cfa71d52a6
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spelling	oai:doaj.org-article:087cf85ea5924b77a3edc2cfa71d52a62021-12-02T11:52:22ZReference genes for gene expression studies in the mouse heart10.1038/s41598-017-00043-92045-2322https://doaj.org/article/087cf85ea5924b77a3edc2cfa71d52a62017-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00043-9https://doaj.org/toc/2045-2322Abstract To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research.Adrián Ruiz-VillalbaAndrea MattiottiQuinn D. GunstSara Cano-BallesterosMaurice J. B. van den HoffJan M. RuijterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrián Ruiz-Villalba Andrea Mattiotti Quinn D. Gunst Sara Cano-Ballesteros Maurice J. B. van den Hoff Jan M. Ruijter Reference genes for gene expression studies in the mouse heart
description	Abstract To be accurate, quantitative Polymerase Chain Reaction (qPCR) studies require a set of stable reference genes for normalization. This is especially critical in cardiac research because of the diversity of the clinical and experimental conditions in the field. We analyzed the stability of previously described as potential reference genes in different subsets of cardiac tissues, each representing a different field in cardiac research. The qPCR dataset was based on 119 different tissue samples derived from cardiac development to pathology in mouse adult hearts. These samples were grouped into 47 tissue types. The stability of 9 candidate genes was analyzed in each of 12 experimental conditions comprising different groupings of these tissue types. Expression stability was determined with the geNorm module of qbase+. This analysis showed that different sets of two or three reference genes are required for analysis of qPCR data in different experimental conditions in murine cardiac research.
format	article
author	Adrián Ruiz-Villalba Andrea Mattiotti Quinn D. Gunst Sara Cano-Ballesteros Maurice J. B. van den Hoff Jan M. Ruijter
author_facet	Adrián Ruiz-Villalba Andrea Mattiotti Quinn D. Gunst Sara Cano-Ballesteros Maurice J. B. van den Hoff Jan M. Ruijter
author_sort	Adrián Ruiz-Villalba
title	Reference genes for gene expression studies in the mouse heart
title_short	Reference genes for gene expression studies in the mouse heart
title_full	Reference genes for gene expression studies in the mouse heart
title_fullStr	Reference genes for gene expression studies in the mouse heart
title_full_unstemmed	Reference genes for gene expression studies in the mouse heart
title_sort	reference genes for gene expression studies in the mouse heart
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/087cf85ea5924b77a3edc2cfa71d52a6
work_keys_str_mv	AT adrianruizvillalba referencegenesforgeneexpressionstudiesinthemouseheart AT andreamattiotti referencegenesforgeneexpressionstudiesinthemouseheart AT quinndgunst referencegenesforgeneexpressionstudiesinthemouseheart AT saracanoballesteros referencegenesforgeneexpressionstudiesinthemouseheart AT mauricejbvandenhoff referencegenesforgeneexpressionstudiesinthemouseheart AT janmruijter referencegenesforgeneexpressionstudiesinthemouseheart
_version_	1718395027299762176

Reference genes for gene expression studies in the mouse heart

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