FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS

Antigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic...

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Autores principales:	A. A. Grazhdantseva, G. F. Sivolobova, M. Major, E. V. Chub, A. N. Shvalov, P. V. Yudin, S. V. Netesov, V. B. Loktev, G. V. Kochneva
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	hepatitis c virus genоtype peptide analysis antigenic determinant Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/088055b801fe4031bb57269c7a4ab8c1
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spelling	oai:doaj.org-article:088055b801fe4031bb57269c7a4ab8c12021-11-18T08:03:37ZFULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS1563-06252313-741X10.15789/1563-0625-2010-1-2-115-124https://doaj.org/article/088055b801fe4031bb57269c7a4ab8c12014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/52https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAntigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic peptide epitopes of Е1 and Е2 proteins common for all HCV genotypes, are located in amino acid positions 250-260, 315-325 (Е1 protein), 390-400 (hypervariable region 1), 430-440, and 680-690 (Е2 protein). The greatest inter-genotypic differences were recorded in positions 280-290, 410-430 and 520-540. A novel antigenic determinant was detected in the region of aa 280-290 of the Е1 protein which was typical only for HCV 2a/2c genotype. A broad variation in the boundaries for the most epitopes suggests a high variability of the Е1 and Е2 viral proteins; however, a similar repertoire of antibodies induced by different HCV genotypes indicates to an opportunity of designing a new generation of cross-reactive HCV vaccines based on mapping of the E1 and E2 antigenic regions.A. A. GrazhdantsevaG. F. SivolobovaM. MajorE. V. ChubA. N. ShvalovP. V. YudinS. V. NetesovV. B. LoktevG. V. KochnevaSPb RAACIarticlehepatitis c virusgenоtypepeptide analysisantigenic determinantImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 1-2, Pp 115-124 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	hepatitis c virus genоtype peptide analysis antigenic determinant Immunologic diseases. Allergy RC581-607
spellingShingle	hepatitis c virus genоtype peptide analysis antigenic determinant Immunologic diseases. Allergy RC581-607 A. A. Grazhdantseva G. F. Sivolobova M. Major E. V. Chub A. N. Shvalov P. V. Yudin S. V. Netesov V. B. Loktev G. V. Kochneva FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
description	Antigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic peptide epitopes of Е1 and Е2 proteins common for all HCV genotypes, are located in amino acid positions 250-260, 315-325 (Е1 protein), 390-400 (hypervariable region 1), 430-440, and 680-690 (Е2 protein). The greatest inter-genotypic differences were recorded in positions 280-290, 410-430 and 520-540. A novel antigenic determinant was detected in the region of aa 280-290 of the Е1 protein which was typical only for HCV 2a/2c genotype. A broad variation in the boundaries for the most epitopes suggests a high variability of the Е1 and Е2 viral proteins; however, a similar repertoire of antibodies induced by different HCV genotypes indicates to an opportunity of designing a new generation of cross-reactive HCV vaccines based on mapping of the E1 and E2 antigenic regions.
format	article
author	A. A. Grazhdantseva G. F. Sivolobova M. Major E. V. Chub A. N. Shvalov P. V. Yudin S. V. Netesov V. B. Loktev G. V. Kochneva
author_facet	A. A. Grazhdantseva G. F. Sivolobova M. Major E. V. Chub A. N. Shvalov P. V. Yudin S. V. Netesov V. B. Loktev G. V. Kochneva
author_sort	A. A. Grazhdantseva
title	FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
title_short	FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
title_full	FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
title_fullStr	FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
title_full_unstemmed	FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
title_sort	full-length peptide assay of antigenic profile of envelope proteins from siberian isolates of hepatitis c virus
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/088055b801fe4031bb57269c7a4ab8c1
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FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS

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