Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue

Modern Pelargonium crispum hybrids (section Pelargonium) show low genetic and phenotypic variation due to the domestication effect. Species of the sections Cortusina, Ligularia, and Pelargonium are potential breeding partners at the diploid level (2n = 2x = 22). Five P. × crispum cultivars were u...

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Autores principales:	Sylvia Plaschil, Holger Budahn, Evelyn Klocke, Michael Wiedemann, Klaus Olbricht
Formato:	article
Lenguaje:	EN
Publicado:	Julius Kühn-Institut 2021
Materias:	Plant culture SB1-1110 Botany QK1-989
Acceso en línea:	https://doaj.org/article/0880f753157245f1b3c38261c5123dfa
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spelling	oai:doaj.org-article:0880f753157245f1b3c38261c5123dfa2021-12-01T10:15:00ZSpontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue10.5073/JABFQ.2021.094.0251613-92161439-040Xhttps://doaj.org/article/0880f753157245f1b3c38261c5123dfa2021-12-01T00:00:00Zhttps://ojs.openagrar.de/index.php/JABFQ/article/view/16353https://doaj.org/toc/1613-9216https://doaj.org/toc/1439-040X Modern Pelargonium crispum hybrids (section Pelargonium) show low genetic and phenotypic variation due to the domestication effect. Species of the sections Cortusina, Ligularia, and Pelargonium are potential breeding partners at the diploid level (2n = 2x = 22). Five P. × crispum cultivars were used as seed parents and pollinated with one genotype of P. grandiflorum (section Pelargonium) and three genotypes of P. fulgidum (section Ligularia). In both combinations, embryo rescue was necessary. Embryos were rescued and cultured on Murashige & Skoog medium supplemented with phytohormones. After callus and adventitious shoot regeneration 15 viable interspecific hybrids were obtained from crossbreeding with P. grandiflorum and 11 intersectional hybrids from crossings with P. fulgidum, respectively. The hybrids were cultivated in the greenhouse until flowering. Their hybrid character was evident due to the intermediate morphological traits. Molecular investigations using dp-RAPD analysis confirmed this. Within the F1 population P. × crispum with P. grandiflorum three hybrids and after crossing with P. fulgidum one hybrid possessed larger flowers and fully developed anthers, respectively. Their ploidy level was confirmed as tetraploid using flow cytometry. Therefore, a spontaneous polyploidisation occurred during in vitro regeneration. The tetraploid F1 hybrids are fertile and could be used for further breeding. Sylvia PlaschilHolger BudahnEvelyn KlockeMichael WiedemannKlaus OlbrichtJulius Kühn-InstitutarticlePlant cultureSB1-1110BotanyQK1-989ENJournal of Applied Botany and Food Quality, Vol 94 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plant culture SB1-1110 Botany QK1-989
spellingShingle	Plant culture SB1-1110 Botany QK1-989 Sylvia Plaschil Holger Budahn Evelyn Klocke Michael Wiedemann Klaus Olbricht Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
description	Modern Pelargonium crispum hybrids (section Pelargonium) show low genetic and phenotypic variation due to the domestication effect. Species of the sections Cortusina, Ligularia, and Pelargonium are potential breeding partners at the diploid level (2n = 2x = 22). Five P. × crispum cultivars were used as seed parents and pollinated with one genotype of P. grandiflorum (section Pelargonium) and three genotypes of P. fulgidum (section Ligularia). In both combinations, embryo rescue was necessary. Embryos were rescued and cultured on Murashige & Skoog medium supplemented with phytohormones. After callus and adventitious shoot regeneration 15 viable interspecific hybrids were obtained from crossbreeding with P. grandiflorum and 11 intersectional hybrids from crossings with P. fulgidum, respectively. The hybrids were cultivated in the greenhouse until flowering. Their hybrid character was evident due to the intermediate morphological traits. Molecular investigations using dp-RAPD analysis confirmed this. Within the F1 population P. × crispum with P. grandiflorum three hybrids and after crossing with P. fulgidum one hybrid possessed larger flowers and fully developed anthers, respectively. Their ploidy level was confirmed as tetraploid using flow cytometry. Therefore, a spontaneous polyploidisation occurred during in vitro regeneration. The tetraploid F1 hybrids are fertile and could be used for further breeding.
format	article
author	Sylvia Plaschil Holger Budahn Evelyn Klocke Michael Wiedemann Klaus Olbricht
author_facet	Sylvia Plaschil Holger Budahn Evelyn Klocke Michael Wiedemann Klaus Olbricht
author_sort	Sylvia Plaschil
title	Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
title_short	Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
title_full	Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
title_fullStr	Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
title_full_unstemmed	Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue
title_sort	spontaneous polyploidisation of interspecific and intersectional pelargonium hybrids during embryo rescue
publisher	Julius Kühn-Institut
publishDate	2021
url	https://doaj.org/article/0880f753157245f1b3c38261c5123dfa
work_keys_str_mv	AT sylviaplaschil spontaneouspolyploidisationofinterspecificandintersectionalpelargoniumhybridsduringembryorescue AT holgerbudahn spontaneouspolyploidisationofinterspecificandintersectionalpelargoniumhybridsduringembryorescue AT evelynklocke spontaneouspolyploidisationofinterspecificandintersectionalpelargoniumhybridsduringembryorescue AT michaelwiedemann spontaneouspolyploidisationofinterspecificandintersectionalpelargoniumhybridsduringembryorescue AT klausolbricht spontaneouspolyploidisationofinterspecificandintersectionalpelargoniumhybridsduringembryorescue
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Spontaneous polyploidisation of interspecific and intersectional Pelargonium hybrids during embryo rescue

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