Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.

MicroRNAs (miRNA) have emerged as key regulators of cell lineage differentiation and cancer. We used precursor miRNA profiling by a novel real-time QPCR method (i) to define progressive stages of endothelial cell transformation cumulating in Kaposi sarcoma (KS) and (ii) to identify specific miRNAs t...

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Autores principales:	Andrea J O'Hara, Pauline Chugh, Ling Wang, Eduardo M Netto, Estrella Luz, William J Harrington, Bruce J Dezube, Blossom Damania, Dirk P Dittmer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0884992e5ef945ab99a608557d48284f
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spelling	oai:doaj.org-article:0884992e5ef945ab99a608557d48284f2021-11-25T05:47:06ZPre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.1553-73661553-737410.1371/journal.ppat.1000389https://doaj.org/article/0884992e5ef945ab99a608557d48284f2009-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19381257/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374MicroRNAs (miRNA) have emerged as key regulators of cell lineage differentiation and cancer. We used precursor miRNA profiling by a novel real-time QPCR method (i) to define progressive stages of endothelial cell transformation cumulating in Kaposi sarcoma (KS) and (ii) to identify specific miRNAs that serve as biomarkers for tumor progression. We were able to compare primary patient biopsies to well-established culture and mouse tumor models. Loss of mir-221 and gain of mir-15 expression demarked the transition from merely immortalized to fully tumorigenic endothelial cells. Mir-140 and Kaposi sarcoma-associated herpesvirus viral miRNAs increased linearly with the degree of transformation. Mir-24 emerged as a biomarker specific for KS.Andrea J O'HaraPauline ChughLing WangEduardo M NettoEstrella LuzWilliam J HarringtonBruce J DezubeBlossom DamaniaDirk P DittmerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 4, p e1000389 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Andrea J O'Hara Pauline Chugh Ling Wang Eduardo M Netto Estrella Luz William J Harrington Bruce J Dezube Blossom Damania Dirk P Dittmer Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
description	MicroRNAs (miRNA) have emerged as key regulators of cell lineage differentiation and cancer. We used precursor miRNA profiling by a novel real-time QPCR method (i) to define progressive stages of endothelial cell transformation cumulating in Kaposi sarcoma (KS) and (ii) to identify specific miRNAs that serve as biomarkers for tumor progression. We were able to compare primary patient biopsies to well-established culture and mouse tumor models. Loss of mir-221 and gain of mir-15 expression demarked the transition from merely immortalized to fully tumorigenic endothelial cells. Mir-140 and Kaposi sarcoma-associated herpesvirus viral miRNAs increased linearly with the degree of transformation. Mir-24 emerged as a biomarker specific for KS.
format	article
author	Andrea J O'Hara Pauline Chugh Ling Wang Eduardo M Netto Estrella Luz William J Harrington Bruce J Dezube Blossom Damania Dirk P Dittmer
author_facet	Andrea J O'Hara Pauline Chugh Ling Wang Eduardo M Netto Estrella Luz William J Harrington Bruce J Dezube Blossom Damania Dirk P Dittmer
author_sort	Andrea J O'Hara
title	Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
title_short	Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
title_full	Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
title_fullStr	Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
title_full_unstemmed	Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.
title_sort	pre-micro rna signatures delineate stages of endothelial cell transformation in kaposi sarcoma.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/0884992e5ef945ab99a608557d48284f
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Pre-micro RNA signatures delineate stages of endothelial cell transformation in Kaposi sarcoma.

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