Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.

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Detalles Bibliográficos
Autores principales:	David J. H. F. Knapp, Yale S. Michaels, Max Jamilly, Quentin R. V. Ferry, Hector Barbosa, Thomas A. Milne, Tudor A. Fulga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/088592c408394975893eeb5eb3ce5546
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spelling	oai:doaj.org-article:088592c408394975893eeb5eb3ce55462021-12-02T16:57:22ZDecoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression10.1038/s41467-019-09148-32041-1723https://doaj.org/article/088592c408394975893eeb5eb3ce55462019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09148-3https://doaj.org/toc/2041-1723The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.David J. H. F. KnappYale S. MichaelsMax JamillyQuentin R. V. FerryHector BarbosaThomas A. MilneTudor A. FulgaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q David J. H. F. Knapp Yale S. Michaels Max Jamilly Quentin R. V. Ferry Hector Barbosa Thomas A. Milne Tudor A. Fulga Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
description	The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.
format	article
author	David J. H. F. Knapp Yale S. Michaels Max Jamilly Quentin R. V. Ferry Hector Barbosa Thomas A. Milne Tudor A. Fulga
author_facet	David J. H. F. Knapp Yale S. Michaels Max Jamilly Quentin R. V. Ferry Hector Barbosa Thomas A. Milne Tudor A. Fulga
author_sort	David J. H. F. Knapp
title	Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
title_short	Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
title_full	Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
title_fullStr	Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
title_full_unstemmed	Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
title_sort	decoupling trna promoter and processing activities enables specific pol-ii cas9 guide rna expression
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/088592c408394975893eeb5eb3ce5546
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Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

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