Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.

Guardado en:
Detalles Bibliográficos
Autores principales: David J. H. F. Knapp, Yale S. Michaels, Max Jamilly, Quentin R. V. Ferry, Hector Barbosa, Thomas A. Milne, Tudor A. Fulga
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
Materias:
Q
Acceso en línea:https://doaj.org/article/088592c408394975893eeb5eb3ce5546
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!