Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt

In recent times, resistant foodborne pathogens, especially of the <i>Campylobacter</i> species, have created several global crises. These crises have been compounded due to the evolution of multidrug-resistant (MDR) bacterial pathogens and the emergence of extensively drug-resistant (XDR...

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Autores principales:	Ahmed M. Ammar, Marwa I. Abd El-Hamid, Rania M. S. El-Malt, Doaa S. Azab, Sarah Albogami, Mohammad M. Al-Sanea, Wafaa E. Soliman, Mohammed M. Ghoneim, Mahmoud M. Bendary
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Campylobacter</i> species PDR FQ resistant <i>gyrA</i> PCR-RFLP Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/089c6ffcd76d4d60af89af1210591886
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spelling	oai:doaj.org-article:089c6ffcd76d4d60af89af12105918862021-11-25T16:23:12ZMolecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt10.3390/antibiotics101113422079-6382https://doaj.org/article/089c6ffcd76d4d60af89af12105918862021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1342https://doaj.org/toc/2079-6382In recent times, resistant foodborne pathogens, especially of the <i>Campylobacter</i> species, have created several global crises. These crises have been compounded due to the evolution of multidrug-resistant (MDR) bacterial pathogens and the emergence of extensively drug-resistant (XDR) and pan-drug-resistant (PDR) strains. Therefore, this study aimed to investigate the development of resistance and the existence of both XDR and PDR among <i>Campylobacter</i> isolates. Moreover, we explored the use of the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique for the detection of fluoroquinolone (FQ)-resistant <i>Campylobacter</i> isolates. A total of 120 <i>Campylobacter</i> isolates were identified depending on both phenotypic and genotypic methods. Of note, cefoxitin and imipenem were the most effective drugs against the investigated <i>Campylobacter</i> isolates. Interestingly, the majority of our isolates (75%) were MDR. Unfortunately, both XDR and PDR isolates were detected in our study with prevalence rates of 20.8% and 4.2%, respectively. All FQ-resistant isolates with ciprofloxacin minimum inhibitory concentrations ≥4 µg/mL were confirmed by the genetic detection of <i>gyrA</i> chromosomal mutation via substitution of threonine at position 86 to isoleucine (Thr-86-to-Ile) using the PCR-RFLP technique. Herein, PCR-RFLP was a more practical and less expensive method used for the detection of FQ resistant isolates. In conclusion, we introduced a fast genetic method for the identification of FQ-resistant isolates to avoid treatment failure through the proper description of antimicrobials.Ahmed M. AmmarMarwa I. Abd El-HamidRania M. S. El-MaltDoaa S. AzabSarah AlbogamiMohammad M. Al-SaneaWafaa E. SolimanMohammed M. GhoneimMahmoud M. BendaryMDPI AGarticle<i>Campylobacter</i> speciesPDRFQ resistant<i>gyrA</i>PCR-RFLPTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1342, p 1342 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Campylobacter</i> species PDR FQ resistant <i>gyrA</i> PCR-RFLP Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Campylobacter</i> species PDR FQ resistant <i>gyrA</i> PCR-RFLP Therapeutics. Pharmacology RM1-950 Ahmed M. Ammar Marwa I. Abd El-Hamid Rania M. S. El-Malt Doaa S. Azab Sarah Albogami Mohammad M. Al-Sanea Wafaa E. Soliman Mohammed M. Ghoneim Mahmoud M. Bendary Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
description	In recent times, resistant foodborne pathogens, especially of the <i>Campylobacter</i> species, have created several global crises. These crises have been compounded due to the evolution of multidrug-resistant (MDR) bacterial pathogens and the emergence of extensively drug-resistant (XDR) and pan-drug-resistant (PDR) strains. Therefore, this study aimed to investigate the development of resistance and the existence of both XDR and PDR among <i>Campylobacter</i> isolates. Moreover, we explored the use of the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique for the detection of fluoroquinolone (FQ)-resistant <i>Campylobacter</i> isolates. A total of 120 <i>Campylobacter</i> isolates were identified depending on both phenotypic and genotypic methods. Of note, cefoxitin and imipenem were the most effective drugs against the investigated <i>Campylobacter</i> isolates. Interestingly, the majority of our isolates (75%) were MDR. Unfortunately, both XDR and PDR isolates were detected in our study with prevalence rates of 20.8% and 4.2%, respectively. All FQ-resistant isolates with ciprofloxacin minimum inhibitory concentrations ≥4 µg/mL were confirmed by the genetic detection of <i>gyrA</i> chromosomal mutation via substitution of threonine at position 86 to isoleucine (Thr-86-to-Ile) using the PCR-RFLP technique. Herein, PCR-RFLP was a more practical and less expensive method used for the detection of FQ resistant isolates. In conclusion, we introduced a fast genetic method for the identification of FQ-resistant isolates to avoid treatment failure through the proper description of antimicrobials.
format	article
author	Ahmed M. Ammar Marwa I. Abd El-Hamid Rania M. S. El-Malt Doaa S. Azab Sarah Albogami Mohammad M. Al-Sanea Wafaa E. Soliman Mohammed M. Ghoneim Mahmoud M. Bendary
author_facet	Ahmed M. Ammar Marwa I. Abd El-Hamid Rania M. S. El-Malt Doaa S. Azab Sarah Albogami Mohammad M. Al-Sanea Wafaa E. Soliman Mohammed M. Ghoneim Mahmoud M. Bendary
author_sort	Ahmed M. Ammar
title	Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
title_short	Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
title_full	Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
title_fullStr	Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
title_full_unstemmed	Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt
title_sort	molecular detection of fluoroquinolone resistance among multidrug-, extensively drug-, and pan-drug-resistant <i>campylobacter</i> species in egypt
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/089c6ffcd76d4d60af89af1210591886
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Molecular Detection of Fluoroquinolone Resistance among Multidrug-, Extensively Drug-, and Pan-Drug-Resistant <i>Campylobacter</i> Species in Egypt

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