Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field

Yaping Yang,1,* Shuangshuang Ren,2,* Xuan Zhang,2 Yijun Yu,1 Chao Liu,3 Jie Yang,2 Leiying Miao1 1Department of Cariology and Endodontics, 2Department of Periodontology, 3Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China *The...

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Autores principales:	Yang Y, Ren S, Zhang X, Yu Y, Liu C, Yang J, Miao L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Ag-Fe3O4 PLGA Dental implants Antibacterial property Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/08a764a21ee74c5b80ab891a7a42c441
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spelling	oai:doaj.org-article:08a764a21ee74c5b80ab891a7a42c4412021-12-02T05:17:05ZSafety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field1178-2013https://doaj.org/article/08a764a21ee74c5b80ab891a7a42c4412018-06-01T00:00:00Zhttps://www.dovepress.com/safety-and-efficacy-of-plgaag-fe3o4-coated-dental-implants-in-inhibiti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yaping Yang,1,* Shuangshuang Ren,2,* Xuan Zhang,2 Yijun Yu,1 Chao Liu,3 Jie Yang,2 Leiying Miao1 1Department of Cariology and Endodontics, 2Department of Periodontology, 3Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China *These authors contributed equally to this work Introduction: The placement of dental implants is performed in a contaminated surgical field in the oral cavity, which may lead to implant failure. Bacterial adhesion and proliferation (Streptococcus mutans, Porphyromonas gingivalis) often lead to implant infections. Although Ag nanoparticles hold great promise for a broad spectrum of antibacterial activities, their runoff from dental implants compromises their antibacterial efficacy and potentially impairs osteoblast proliferation. Thus, this aspect remains a primary challenge and should be controlled. Materials and methods: In this study, PLGA(Ag-Fe3O4) was modified on the implanted tooth surface and was characterized by transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The magnetic and antibacterial properties were also determined. Results: Results showed that Ag successfully bonded with Fe3O4, and Ag-Fe3O4 not only exerted superparamagnetism but also exhibited antibacterial activity almost identical to silver nanoparticles (nano-Ag). The PLGA(Ag-Fe3O4) coating could significantly maintain the antibacterial activity and avoid bacterial adhesion to the implant. Compared with the blank control group, PLGA(Ag-Fe3O4) under magnetic field-coated samples had a significantly lower amount of colonized S. mutans (P<0.01). Osteoblast proliferation results showed that the coated samples did not exhibit cytotoxicity and could promote osteoblast proliferation as shown by MTT, alkaline phosphatase, and the nucleolar organizer region count. Conclusion: We developed a novel Ag biologically compatible nanoparticle in this study without compromising the nano-Ag antibacterial activity, which provided continuous antibacterial action. Keywords: Ag-Fe3O4, PLGA, dental implants, antibacterial propertyYang YRen SZhang XYu YLiu CYang JMiao LDove Medical PressarticleAg-Fe3O4PLGADental implantsAntibacterial propertyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 3751-3762 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ag-Fe3O4 PLGA Dental implants Antibacterial property Medicine (General) R5-920
spellingShingle	Ag-Fe3O4 PLGA Dental implants Antibacterial property Medicine (General) R5-920 Yang Y Ren S Zhang X Yu Y Liu C Yang J Miao L Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
description	Yaping Yang,1,* Shuangshuang Ren,2,* Xuan Zhang,2 Yijun Yu,1 Chao Liu,3 Jie Yang,2 Leiying Miao1 1Department of Cariology and Endodontics, 2Department of Periodontology, 3Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China *These authors contributed equally to this work Introduction: The placement of dental implants is performed in a contaminated surgical field in the oral cavity, which may lead to implant failure. Bacterial adhesion and proliferation (Streptococcus mutans, Porphyromonas gingivalis) often lead to implant infections. Although Ag nanoparticles hold great promise for a broad spectrum of antibacterial activities, their runoff from dental implants compromises their antibacterial efficacy and potentially impairs osteoblast proliferation. Thus, this aspect remains a primary challenge and should be controlled. Materials and methods: In this study, PLGA(Ag-Fe3O4) was modified on the implanted tooth surface and was characterized by transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The magnetic and antibacterial properties were also determined. Results: Results showed that Ag successfully bonded with Fe3O4, and Ag-Fe3O4 not only exerted superparamagnetism but also exhibited antibacterial activity almost identical to silver nanoparticles (nano-Ag). The PLGA(Ag-Fe3O4) coating could significantly maintain the antibacterial activity and avoid bacterial adhesion to the implant. Compared with the blank control group, PLGA(Ag-Fe3O4) under magnetic field-coated samples had a significantly lower amount of colonized S. mutans (P<0.01). Osteoblast proliferation results showed that the coated samples did not exhibit cytotoxicity and could promote osteoblast proliferation as shown by MTT, alkaline phosphatase, and the nucleolar organizer region count. Conclusion: We developed a novel Ag biologically compatible nanoparticle in this study without compromising the nano-Ag antibacterial activity, which provided continuous antibacterial action. Keywords: Ag-Fe3O4, PLGA, dental implants, antibacterial property
format	article
author	Yang Y Ren S Zhang X Yu Y Liu C Yang J Miao L
author_facet	Yang Y Ren S Zhang X Yu Y Liu C Yang J Miao L
author_sort	Yang Y
title	Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
title_short	Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
title_full	Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
title_fullStr	Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
title_full_unstemmed	Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
title_sort	safety and efficacy of plga(ag-fe3o4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/08a764a21ee74c5b80ab891a7a42c441
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Safety and efficacy of PLGA(Ag-Fe3O4)-coated dental implants in inhibiting bacteria adherence and osteogenic inducement under a magnetic field

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