Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in...

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Autores principales:	Greta Labutytė, Simona Povilonienė, Eugenijus Šimoliūnas, Dovydas Gabrielaitis, Martynas Skapas, Algirdas Noreika, Rolandas Meškys, Vida Časaitė
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacteriophage vB_KleM-RaK2 tail sheath protein nanotube green fluorescent protein mCherry YqfB Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/08ae77462d274fb7b2f8279b54ebfe09
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spelling	oai:doaj.org-article:08ae77462d274fb7b2f8279b54ebfe092021-11-25T18:31:51ZFunctionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein10.3390/nano111130312079-4991https://doaj.org/article/08ae77462d274fb7b2f8279b54ebfe092021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/3031https://doaj.org/toc/2079-4991We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in <i>E. coli</i> BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041Δ200GFP and 041Δ200mCherry were coexpressed in <i>E. coli</i> BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041Δ200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of <i>N</i><sup>4</sup>-acetyl-2′-deoxycytidine. The enzymatic properties of 041Δ200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts.Greta LabutytėSimona PovilonienėEugenijus ŠimoliūnasDovydas GabrielaitisMartynas SkapasAlgirdas NoreikaRolandas MeškysVida ČasaitėMDPI AGarticlebacteriophage vB_KleM-RaK2tail sheath proteinnanotubegreen fluorescent proteinmCherryYqfBChemistryQD1-999ENNanomaterials, Vol 11, Iss 3031, p 3031 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage vB_KleM-RaK2 tail sheath protein nanotube green fluorescent protein mCherry YqfB Chemistry QD1-999
spellingShingle	bacteriophage vB_KleM-RaK2 tail sheath protein nanotube green fluorescent protein mCherry YqfB Chemistry QD1-999 Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
description	We report on the construction of functionalized nanotubes based on tail sheath protein 041 from vB_KleM-RaK2 bacteriophage. The truncated 041 protein (041Δ200) was fused with fluorescent proteins GFP and mCherry or amidohydrolase YqfB. The generated chimeric proteins were successfully synthesized in <i>E. coli</i> BL21 (DE3) cells and self-assembled into tubular structures. We detected the fluorescence of the structures, which was confirmed by stimulated emission depletion microscopy. When 041Δ200GFP and 041Δ200mCherry were coexpressed in <i>E. coli</i> BL21 (DE3) cells, the formed nanotubes generated Förster resonance energy transfer, indicating that both fluorescent proteins assemble into a single nanotube. Chimeric 041Δ200YqfB nanotubes possessed an enzymatic activity, which was confirmed by hydrolysis of <i>N</i><sup>4</sup>-acetyl-2′-deoxycytidine. The enzymatic properties of 041Δ200YqfB were similar to those of a free wild-type YqfB. Hence, we conclude that 041-based chimeric nanotubes have the potential for the development of delivery vehicles and targeted imaging and are applicable as scaffolds for biocatalysts.
format	article
author	Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė
author_facet	Greta Labutytė Simona Povilonienė Eugenijus Šimoliūnas Dovydas Gabrielaitis Martynas Skapas Algirdas Noreika Rolandas Meškys Vida Časaitė
author_sort	Greta Labutytė
title	Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
title_short	Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
title_full	Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
title_fullStr	Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
title_full_unstemmed	Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein
title_sort	functionalized protein nanotubes based on the bacteriophage vb_klem-rak2 tail sheath protein
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/08ae77462d274fb7b2f8279b54ebfe09
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Functionalized Protein Nanotubes Based on the Bacteriophage vB_KleM-RaK2 Tail Sheath Protein

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