Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]

Estrogens are important regulators of body physiology and have major effects on metabolism, bone, the immune- and central nervous systems. The specific mechanisms underlying the effects of estrogens on various cells, tissues and organs are unclear and mouse models constitute a powerful experimental...

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Autores principales:	Carmen Corciulo, Julia M. Scheffler, Karin L. Gustafsson, Christina Drevinge, Piotr Humeniuk, Alicia M. del Carpio Pons, Matti Poutanen, Claes Ohlsson, Marie K. Lagerquist, Ulrika Islander
Formato:	article
Lenguaje:	EN
Publicado:	F1000 Research Ltd 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08b12fa6283b4760a790738d95c9a0db
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spelling	oai:doaj.org-article:08b12fa6283b4760a790738d95c9a0db2021-11-29T14:03:15ZPulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]2046-140210.12688/f1000research.54501.1https://doaj.org/article/08b12fa6283b4760a790738d95c9a0db2021-08-01T00:00:00Zhttps://f1000research.com/articles/10-809/v1https://doaj.org/toc/2046-1402Estrogens are important regulators of body physiology and have major effects on metabolism, bone, the immune- and central nervous systems. The specific mechanisms underlying the effects of estrogens on various cells, tissues and organs are unclear and mouse models constitute a powerful experimental tool to define the physiological and pathological properties of estrogens. Menopause can be mimicked in animal models by surgical removal of the ovaries and replacement therapy with 17β-estradiol in ovariectomized (OVX) mice is a common technique used to determine specific effects of the hormone. However, these studies are complicated by the non-monotonic dose-response of estradiol, when given as therapy. Increased knowledge of how to distribute estradiol in terms of solvent, dose, and administration frequency, is required in order to accurately mimic physiological conditions in studies where estradiol treatment is performed. In this study, mice were OVX and treated with physiological doses of 17β-estradiol-3-benzoate (E2) dissolved in miglyol or PBS. Subcutaneous injections were performed every 4 days to resemble the estrus cycle in mice. Results show that OVX induces an osteoporotic phenotype, fat accumulation and impairment of the locomotor ability, as expected. Pulsed administration of physiological doses of E2 dissolved in miglyol rescues the phenotypes induced by OVX. However, when E2 is dissolved in PBS the effects are less pronounced, possibly due to rapid wash out of the steroid.Carmen CorciuloJulia M. SchefflerKarin L. GustafssonChristina DrevingePiotr HumeniukAlicia M. del Carpio PonsMatti PoutanenClaes OhlssonMarie K. LagerquistUlrika IslanderF1000 Research LtdarticleMedicineRScienceQENF1000Research, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carmen Corciulo Julia M. Scheffler Karin L. Gustafsson Christina Drevinge Piotr Humeniuk Alicia M. del Carpio Pons Matti Poutanen Claes Ohlsson Marie K. Lagerquist Ulrika Islander Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
description	Estrogens are important regulators of body physiology and have major effects on metabolism, bone, the immune- and central nervous systems. The specific mechanisms underlying the effects of estrogens on various cells, tissues and organs are unclear and mouse models constitute a powerful experimental tool to define the physiological and pathological properties of estrogens. Menopause can be mimicked in animal models by surgical removal of the ovaries and replacement therapy with 17β-estradiol in ovariectomized (OVX) mice is a common technique used to determine specific effects of the hormone. However, these studies are complicated by the non-monotonic dose-response of estradiol, when given as therapy. Increased knowledge of how to distribute estradiol in terms of solvent, dose, and administration frequency, is required in order to accurately mimic physiological conditions in studies where estradiol treatment is performed. In this study, mice were OVX and treated with physiological doses of 17β-estradiol-3-benzoate (E2) dissolved in miglyol or PBS. Subcutaneous injections were performed every 4 days to resemble the estrus cycle in mice. Results show that OVX induces an osteoporotic phenotype, fat accumulation and impairment of the locomotor ability, as expected. Pulsed administration of physiological doses of E2 dissolved in miglyol rescues the phenotypes induced by OVX. However, when E2 is dissolved in PBS the effects are less pronounced, possibly due to rapid wash out of the steroid.
format	article
author	Carmen Corciulo Julia M. Scheffler Karin L. Gustafsson Christina Drevinge Piotr Humeniuk Alicia M. del Carpio Pons Matti Poutanen Claes Ohlsson Marie K. Lagerquist Ulrika Islander
author_facet	Carmen Corciulo Julia M. Scheffler Karin L. Gustafsson Christina Drevinge Piotr Humeniuk Alicia M. del Carpio Pons Matti Poutanen Claes Ohlsson Marie K. Lagerquist Ulrika Islander
author_sort	Carmen Corciulo
title	Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
title_short	Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
title_full	Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
title_fullStr	Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
title_full_unstemmed	Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
title_sort	pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]
publisher	F1000 Research Ltd
publishDate	2021
url	https://doaj.org/article/08b12fa6283b4760a790738d95c9a0db
work_keys_str_mv	AT carmencorciulo pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT juliamscheffler pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT karinlgustafsson pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT christinadrevinge pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT piotrhumeniuk pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT aliciamdelcarpiopons pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT mattipoutanen pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT claesohlsson pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT marieklagerquist pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved AT ulrikaislander pulsedadministrationforphysiologicalestrogenreplacementinmiceversion1peerreview2approved
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Pulsed administration for physiological estrogen replacement in mice [version 1; peer review: 2 approved]

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