Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies
Exercise initiates systemic adaptation to promote health and prevent various lifestyle-related chronic diseases. Emerging evidence suggests that circulating exosomes mediate some of the beneficial effects of exercise via the transfer of microRNAs between tissues. Yet to date, a comprehensive profile...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:08b1c3f2a1d54313957f0e885224b4a62021-11-30T10:18:33ZPhysical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies1664-042X10.3389/fphys.2021.658218https://doaj.org/article/08b1c3f2a1d54313957f0e885224b4a62021-08-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.658218/fullhttps://doaj.org/toc/1664-042XExercise initiates systemic adaptation to promote health and prevent various lifestyle-related chronic diseases. Emerging evidence suggests that circulating exosomes mediate some of the beneficial effects of exercise via the transfer of microRNAs between tissues. Yet to date, a comprehensive profile of the exosomal miRNA (exomiR) content released following short-term (0.5 year in this study) and long-term (25 + years in this study) regular bouts of exercise is still lacking. However, a better understanding of these miRNA species would assist in clarifying the role of regular exercise at the molecular level in the prevention of chronic diseases. In the present pilot studies we analyzed serum exomiR expression in healthy young, sedentary participants (n = 14; age: 23 ± 2 years) at baseline and following a half year-long moderate-intensity regular exercise training. We also analyzed serum exomiR expression in older, healthy trained participants (seniors, n = 11; age: 62 ± 6 years) who engaged in endurance activities for at least 25 years. Following the isolation and enrichment of serum exosomes using Total Exosome Isolation Reagent (TEI) their exomiR levels were determined using the amplification-free Nanostring platform. Hierarchical cluster analysis revealed that the majority of exomiRs overlap for short-term (0.5 year in this study) and long-term (25 + years in this study) regular bouts of exercise. The top 12 significantly altered exomiRs (let-7a-5p; let-7g-5p; miR-130a-3p; miR-142-3p; miR-150-5p; miR-15a-5p; miR-15b-5p; miR-199a-3p; miR-199b-3p; miR-223-3p; miR-23a-3p, and miR-451a-3p) were used for further evaluation. According to KEGG pathway analysis a large portion of the exomiRs target chronic diseases including cancer, neurodegenerative and metabolic diseases, and viral infections. Our results provide evidence that exosomal miRNA modulation is the molecular mechanism through which regular exercise prevents various chronic diseases. The possibility of using such exomiRs to target diseases is of great interest. While further validation is needed, our comprehensive exomiR study presents, for the first time, the disease-preventive molecular pattern of both short and long-term regular exercise.Kitti GaraiKitti GaraiZoltan AdamZoltan AdamRobert HerczegKrisztina BanfaiKrisztina BanfaiAdam GyebrovszkiAttila GyeneseiJudit E. PongraczJudit E. PongraczMarta WilhelmKrisztian KvellKrisztian KvellFrontiers Media S.A.articleregular exerciseexosomemiRNAchronic diseasepreventionPhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021) |
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regular exercise exosome miRNA chronic disease prevention Physiology QP1-981 |
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regular exercise exosome miRNA chronic disease prevention Physiology QP1-981 Kitti Garai Kitti Garai Zoltan Adam Zoltan Adam Robert Herczeg Krisztina Banfai Krisztina Banfai Adam Gyebrovszki Attila Gyenesei Judit E. Pongracz Judit E. Pongracz Marta Wilhelm Krisztian Kvell Krisztian Kvell Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| description |
Exercise initiates systemic adaptation to promote health and prevent various lifestyle-related chronic diseases. Emerging evidence suggests that circulating exosomes mediate some of the beneficial effects of exercise via the transfer of microRNAs between tissues. Yet to date, a comprehensive profile of the exosomal miRNA (exomiR) content released following short-term (0.5 year in this study) and long-term (25 + years in this study) regular bouts of exercise is still lacking. However, a better understanding of these miRNA species would assist in clarifying the role of regular exercise at the molecular level in the prevention of chronic diseases. In the present pilot studies we analyzed serum exomiR expression in healthy young, sedentary participants (n = 14; age: 23 ± 2 years) at baseline and following a half year-long moderate-intensity regular exercise training. We also analyzed serum exomiR expression in older, healthy trained participants (seniors, n = 11; age: 62 ± 6 years) who engaged in endurance activities for at least 25 years. Following the isolation and enrichment of serum exosomes using Total Exosome Isolation Reagent (TEI) their exomiR levels were determined using the amplification-free Nanostring platform. Hierarchical cluster analysis revealed that the majority of exomiRs overlap for short-term (0.5 year in this study) and long-term (25 + years in this study) regular bouts of exercise. The top 12 significantly altered exomiRs (let-7a-5p; let-7g-5p; miR-130a-3p; miR-142-3p; miR-150-5p; miR-15a-5p; miR-15b-5p; miR-199a-3p; miR-199b-3p; miR-223-3p; miR-23a-3p, and miR-451a-3p) were used for further evaluation. According to KEGG pathway analysis a large portion of the exomiRs target chronic diseases including cancer, neurodegenerative and metabolic diseases, and viral infections. Our results provide evidence that exosomal miRNA modulation is the molecular mechanism through which regular exercise prevents various chronic diseases. The possibility of using such exomiRs to target diseases is of great interest. While further validation is needed, our comprehensive exomiR study presents, for the first time, the disease-preventive molecular pattern of both short and long-term regular exercise. |
| format |
article |
| author |
Kitti Garai Kitti Garai Zoltan Adam Zoltan Adam Robert Herczeg Krisztina Banfai Krisztina Banfai Adam Gyebrovszki Attila Gyenesei Judit E. Pongracz Judit E. Pongracz Marta Wilhelm Krisztian Kvell Krisztian Kvell |
| author_facet |
Kitti Garai Kitti Garai Zoltan Adam Zoltan Adam Robert Herczeg Krisztina Banfai Krisztina Banfai Adam Gyebrovszki Attila Gyenesei Judit E. Pongracz Judit E. Pongracz Marta Wilhelm Krisztian Kvell Krisztian Kvell |
| author_sort |
Kitti Garai |
| title |
Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| title_short |
Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| title_full |
Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| title_fullStr |
Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| title_full_unstemmed |
Physical Activity as a Preventive Lifestyle Intervention Acts Through Specific Exosomal miRNA Species—Evidence From Human Short- and Long-Term Pilot Studies |
| title_sort |
physical activity as a preventive lifestyle intervention acts through specific exosomal mirna species—evidence from human short- and long-term pilot studies |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/08b1c3f2a1d54313957f0e885224b4a6 |
| work_keys_str_mv |
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