Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin

Colistin is being considered as “the last ditch” treatment in many infections caused by Gram-negative stains. However, colistin is becoming increasingly invalid in treating patients who are infected with colistin-resistant Escherichia coli (E. coli) and Klebsiella Pneumoniae (K. pneumoniae). To cope...

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Autores principales:	Yishuai Lin, Ying Zhang, Shixing Liu, Dandan Ye, Liqiong Chen, Na Huang, Weiliang Zeng, Wenli Liao, Yizhou Zhan, Tieli Zhou, Jianming Cao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Escherichia coli klebsiella pneumoniae quercetin synergy mechanism colistinresistance Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/08b412eec8ce4c58b0da4762849eb733
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spelling	oai:doaj.org-article:08b412eec8ce4c58b0da4762849eb7332021-12-01T01:30:48ZQuercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin2296-264610.3389/fchem.2021.795150https://doaj.org/article/08b412eec8ce4c58b0da4762849eb7332021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.795150/fullhttps://doaj.org/toc/2296-2646Colistin is being considered as “the last ditch” treatment in many infections caused by Gram-negative stains. However, colistin is becoming increasingly invalid in treating patients who are infected with colistin-resistant Escherichia coli (E. coli) and Klebsiella Pneumoniae (K. pneumoniae). To cope with the continuous emergence of colistin resistance, the development of new drugs and therapies is highly imminent. Herein, in this work, we surprisingly found that the combination of quercetin with colistin could efficiently and synergistically eradicate the colistin-resistant E. coli and K. pneumoniae, as confirmed by the synergy checkboard and time-kill assay. Mechanismly, the treatment of quercetin combined with colistin could significantly downregulate the expression of mcr-1 and mgrB that are responsible for colistin-resistance, synergistically enhancing the bacterial cell membrane damage efficacy of colistin. The colistin/quercetin combination was notably efficient in eradicating the colistin-resistant E. coli and K. pneumoniae both in vitro and in vivo. Therefore, our results may provide an efficient alternative pathway against colistin-resistant E. coli and K. pneumoniae infections.Yishuai LinYing ZhangShixing LiuDandan YeLiqiong ChenNa HuangWeiliang ZengWenli LiaoYizhou ZhanTieli ZhouJianming CaoFrontiers Media S.A.articleEscherichia coliklebsiella pneumoniaequercetinsynergy mechanismcolistinresistanceChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli klebsiella pneumoniae quercetin synergy mechanism colistinresistance Chemistry QD1-999
spellingShingle	Escherichia coli klebsiella pneumoniae quercetin synergy mechanism colistinresistance Chemistry QD1-999 Yishuai Lin Ying Zhang Shixing Liu Dandan Ye Liqiong Chen Na Huang Weiliang Zeng Wenli Liao Yizhou Zhan Tieli Zhou Jianming Cao Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
description	Colistin is being considered as “the last ditch” treatment in many infections caused by Gram-negative stains. However, colistin is becoming increasingly invalid in treating patients who are infected with colistin-resistant Escherichia coli (E. coli) and Klebsiella Pneumoniae (K. pneumoniae). To cope with the continuous emergence of colistin resistance, the development of new drugs and therapies is highly imminent. Herein, in this work, we surprisingly found that the combination of quercetin with colistin could efficiently and synergistically eradicate the colistin-resistant E. coli and K. pneumoniae, as confirmed by the synergy checkboard and time-kill assay. Mechanismly, the treatment of quercetin combined with colistin could significantly downregulate the expression of mcr-1 and mgrB that are responsible for colistin-resistance, synergistically enhancing the bacterial cell membrane damage efficacy of colistin. The colistin/quercetin combination was notably efficient in eradicating the colistin-resistant E. coli and K. pneumoniae both in vitro and in vivo. Therefore, our results may provide an efficient alternative pathway against colistin-resistant E. coli and K. pneumoniae infections.
format	article
author	Yishuai Lin Ying Zhang Shixing Liu Dandan Ye Liqiong Chen Na Huang Weiliang Zeng Wenli Liao Yizhou Zhan Tieli Zhou Jianming Cao
author_facet	Yishuai Lin Ying Zhang Shixing Liu Dandan Ye Liqiong Chen Na Huang Weiliang Zeng Wenli Liao Yizhou Zhan Tieli Zhou Jianming Cao
author_sort	Yishuai Lin
title	Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
title_short	Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
title_full	Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
title_fullStr	Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
title_full_unstemmed	Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin
title_sort	quercetin rejuvenates sensitization of colistin-resistant escherichia coli and klebsiella pneumoniae clinical isolates to colistin
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/08b412eec8ce4c58b0da4762849eb733
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Quercetin Rejuvenates Sensitization of Colistin-Resistant Escherichia coli and Klebsiella Pneumoniae Clinical Isolates to Colistin

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