Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns

Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation...

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Autores principales:	Yesenia Brito‐Pérez, Rodrigo T. Camacho‐Pacheco, Noemi Plazola‐Camacho, Diana Soriano‐Becerril, Irma A. Coronado‐Zarco, Gabriela Arreola‐Ramírez, Gabriela González‐Pérez, Alma Herrera‐Salazar, Julio Flores‐González, Mextli Y. Bermejo‐Haro, Brenda G. Casorla‐Cervantes, Ismael A. Soto‐López, Jessica Hernández‐Pineda, Claudia Sandoval‐Montes, Sandra Rodríguez‐Martínez, Ricardo Figueroa‐Damian, Ismael Mancilla‐Herrera
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	HIV‐exposed uninfected newborns Th cells Th1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/08b8026913fc4464bf52d9b69d5fc5c2
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spelling	oai:doaj.org-article:08b8026913fc4464bf52d9b69d5fc5c22021-11-12T19:57:15ZImpaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns2050-452710.1002/iid3.507https://doaj.org/article/08b8026913fc4464bf52d9b69d5fc5c22021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.507https://doaj.org/toc/2050-4527Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation markers have been found in HEU newborns, suggesting alterations in T cell ontogeny and differentiation. However, little is known about the ability of these cells to produce specialized Th responses from CD4+ T cells. Method To characterize the Th cell profile, we evaluated the frequency of Th1 (CD183+CD194−CD196−/CXCR3+CCR4−CCR6−), Th2 (CD183−CD194+CD196−/CXCR3−CCR4+CCR6−), Th17 (CD183−CD194+CD196+/CXCR3−CCR4+CCR6+), and CD4+CD25++ blood T‐cell phenotypes in 50 HEU and 25 HUU newborns. Early activation markers on CD4+ T cells and the Th cytokine profile produced from mononuclear cells under polyclonal T cell stimulation were also studied. Additionally, we probed the ability of CD4+ T cells to differentiate into interferon (IFN)‐γ‐producing Th1 CD4+ T cells in vitro. Results Lower percentages of differentiated Th1, Th2, Th17, and CD4+CD25++ T cells were found in blood from HEU newborns than in blood from HUU newborns. However, polyclonally stimulated Th cells showed a similar ability to express CD69 and CD279 but produced less secreted interleukin (IL)‐2 and IL‐4. Interestingly, under Th1 differentiation conditions, the percentages of CD4+IFN‐γ+ T cells and soluble IFN‐γ were higher in HEU newborns than in HUU newborns. Conclusion HEU neonates are born with reduced proportions of differentiated Th1/Th2/Th17 and CD4+CD25++ T cells, but the intrinsic abilities of CD4+ T cells to acquire a Th1 profile are not affected by the adverse maternal milieu during development.Yesenia Brito‐PérezRodrigo T. Camacho‐PachecoNoemi Plazola‐CamachoDiana Soriano‐BecerrilIrma A. Coronado‐ZarcoGabriela Arreola‐RamírezGabriela González‐PérezAlma Herrera‐SalazarJulio Flores‐GonzálezMextli Y. Bermejo‐HaroBrenda G. Casorla‐CervantesIsmael A. Soto‐LópezJessica Hernández‐PinedaClaudia Sandoval‐MontesSandra Rodríguez‐MartínezRicardo Figueroa‐DamianIsmael Mancilla‐HerreraWileyarticleHIV‐exposed uninfected newbornsTh cellsTh1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiationImmunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1541-1553 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV‐exposed uninfected newborns Th cells Th1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation Immunologic diseases. Allergy RC581-607
spellingShingle	HIV‐exposed uninfected newborns Th cells Th1/Th2/Th17/Treg/CD4+CD25++ T cells and Th1 differentiation Immunologic diseases. Allergy RC581-607 Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
description	Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months of life compared to HIV‐unexposed uninfected (HUU) newborns. Alterations in thymic mass, amounts of T helper (Th) cells, T‐cell receptor diversity, and activation markers have been found in HEU newborns, suggesting alterations in T cell ontogeny and differentiation. However, little is known about the ability of these cells to produce specialized Th responses from CD4+ T cells. Method To characterize the Th cell profile, we evaluated the frequency of Th1 (CD183+CD194−CD196−/CXCR3+CCR4−CCR6−), Th2 (CD183−CD194+CD196−/CXCR3−CCR4+CCR6−), Th17 (CD183−CD194+CD196+/CXCR3−CCR4+CCR6+), and CD4+CD25++ blood T‐cell phenotypes in 50 HEU and 25 HUU newborns. Early activation markers on CD4+ T cells and the Th cytokine profile produced from mononuclear cells under polyclonal T cell stimulation were also studied. Additionally, we probed the ability of CD4+ T cells to differentiate into interferon (IFN)‐γ‐producing Th1 CD4+ T cells in vitro. Results Lower percentages of differentiated Th1, Th2, Th17, and CD4+CD25++ T cells were found in blood from HEU newborns than in blood from HUU newborns. However, polyclonally stimulated Th cells showed a similar ability to express CD69 and CD279 but produced less secreted interleukin (IL)‐2 and IL‐4. Interestingly, under Th1 differentiation conditions, the percentages of CD4+IFN‐γ+ T cells and soluble IFN‐γ were higher in HEU newborns than in HUU newborns. Conclusion HEU neonates are born with reduced proportions of differentiated Th1/Th2/Th17 and CD4+CD25++ T cells, but the intrinsic abilities of CD4+ T cells to acquire a Th1 profile are not affected by the adverse maternal milieu during development.
format	article
author	Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera
author_facet	Yesenia Brito‐Pérez Rodrigo T. Camacho‐Pacheco Noemi Plazola‐Camacho Diana Soriano‐Becerril Irma A. Coronado‐Zarco Gabriela Arreola‐Ramírez Gabriela González‐Pérez Alma Herrera‐Salazar Julio Flores‐González Mextli Y. Bermejo‐Haro Brenda G. Casorla‐Cervantes Ismael A. Soto‐López Jessica Hernández‐Pineda Claudia Sandoval‐Montes Sandra Rodríguez‐Martínez Ricardo Figueroa‐Damian Ismael Mancilla‐Herrera
author_sort	Yesenia Brito‐Pérez
title	Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
title_short	Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
title_full	Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
title_fullStr	Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
title_full_unstemmed	Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
title_sort	impaired t helper cell responses in human immunodeficiency virus‐exposed uninfected newborns
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/08b8026913fc4464bf52d9b69d5fc5c2
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Impaired T helper cell responses in human immunodeficiency virus‐exposed uninfected newborns

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