Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells

Objective To investigate Krüppel-like factor 17 ( KLF17 ) expression in normal and gastric cancer tissues and cell lines. Methods Levels of KLF17 mRNA and protein in GES-1 normal gastric mucosal cells, and NCI-N87, SGC-7901, BGC-823 and HGC-27 gastric cancer cells were analysed by quantitative polym...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shaoyi Li, Yong Li, Bibo Tan, Zhaojie An
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/08b93552f13b46e392200112bc080986
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:08b93552f13b46e392200112bc080986
record_format	dspace
spelling	oai:doaj.org-article:08b93552f13b46e392200112bc0809862021-11-06T00:03:52ZEffect of overexpression on epithelial–mesenchymal transition of gastric cancer cells1473-230010.1177/03000605211051581https://doaj.org/article/08b93552f13b46e392200112bc0809862021-11-01T00:00:00Zhttps://doi.org/10.1177/03000605211051581https://doaj.org/toc/1473-2300Objective To investigate Krüppel-like factor 17 ( KLF17 ) expression in normal and gastric cancer tissues and cell lines. Methods Levels of KLF17 mRNA and protein in GES-1 normal gastric mucosal cells, and NCI-N87, SGC-7901, BGC-823 and HGC-27 gastric cancer cells were analysed by quantitative polymerase chain reaction (qPCR) and western blot. Differences in KLF17 expression between gastric cancer and adjacent tissues were analysed by qPCR and immunohistochemistry. Invasion/migration effects of KLF17 overexpression in BGC-823 and HGC-27 cells were analysed by wound-healing and Transwell chamber assays. Changes in expression of KLF17 and epithelial–mesenchymal transition (EMT)-related genes (matrix metalloproteinase [MMP]-9, vimentin and E-cadherin) were analysed in BGC-823 and HGC-27 cells before and after transfection using qPCR and western blot. Transforming growth factor (TGF)-β1, Smad family member (Smad)2/3 and phosphorylated-Smad2/3 levels in BGC-823 and HGC-27 cells were assessed by qPCR and western blot. Results KLF17 expression was lower in gastric cancer versus adjacent tissues, and in gastric cancer cell lines versus GES-1 normal gastric mucosal cells, and was positively correlated with degree of cancer-cell differentiation. Wound-healing and Transwell assays showed decreased migration and invasion ability of BGC-823 and HGC-27 cells transfected to overexpress KLF17 . KLF17 overexpression was associated with decreased MMP-9 and vimentin in BGC-823 and HGC-27 cancer cells, and increased KLF17 and E-cadherin. KLF17 overexpression also resulted in decreased levels of TGF-β1 and p-Smad2/3 in BGC-823 and HGC-27 cancer cells. Conclusion KLF17 is poorly expressed in gastric cancer tissues and cell lines. KLF17 overexpression might inhibit EMT via the TGF-β/Smad pathway, thereby reducing gastric cancer cell invasion and migration. Therefore, KLF17 may become a novel target for treating gastric cancer.Shaoyi LiYong LiBibo TanZhaojie AnSAGE PublishingarticleMedicine (General)R5-920ENJournal of International Medical Research, Vol 49 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Shaoyi Li Yong Li Bibo Tan Zhaojie An Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
description	Objective To investigate Krüppel-like factor 17 ( KLF17 ) expression in normal and gastric cancer tissues and cell lines. Methods Levels of KLF17 mRNA and protein in GES-1 normal gastric mucosal cells, and NCI-N87, SGC-7901, BGC-823 and HGC-27 gastric cancer cells were analysed by quantitative polymerase chain reaction (qPCR) and western blot. Differences in KLF17 expression between gastric cancer and adjacent tissues were analysed by qPCR and immunohistochemistry. Invasion/migration effects of KLF17 overexpression in BGC-823 and HGC-27 cells were analysed by wound-healing and Transwell chamber assays. Changes in expression of KLF17 and epithelial–mesenchymal transition (EMT)-related genes (matrix metalloproteinase [MMP]-9, vimentin and E-cadherin) were analysed in BGC-823 and HGC-27 cells before and after transfection using qPCR and western blot. Transforming growth factor (TGF)-β1, Smad family member (Smad)2/3 and phosphorylated-Smad2/3 levels in BGC-823 and HGC-27 cells were assessed by qPCR and western blot. Results KLF17 expression was lower in gastric cancer versus adjacent tissues, and in gastric cancer cell lines versus GES-1 normal gastric mucosal cells, and was positively correlated with degree of cancer-cell differentiation. Wound-healing and Transwell assays showed decreased migration and invasion ability of BGC-823 and HGC-27 cells transfected to overexpress KLF17 . KLF17 overexpression was associated with decreased MMP-9 and vimentin in BGC-823 and HGC-27 cancer cells, and increased KLF17 and E-cadherin. KLF17 overexpression also resulted in decreased levels of TGF-β1 and p-Smad2/3 in BGC-823 and HGC-27 cancer cells. Conclusion KLF17 is poorly expressed in gastric cancer tissues and cell lines. KLF17 overexpression might inhibit EMT via the TGF-β/Smad pathway, thereby reducing gastric cancer cell invasion and migration. Therefore, KLF17 may become a novel target for treating gastric cancer.
format	article
author	Shaoyi Li Yong Li Bibo Tan Zhaojie An
author_facet	Shaoyi Li Yong Li Bibo Tan Zhaojie An
author_sort	Shaoyi Li
title	Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
title_short	Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
title_full	Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
title_fullStr	Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
title_full_unstemmed	Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
title_sort	effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/08b93552f13b46e392200112bc080986
work_keys_str_mv	AT shaoyili effectofoverexpressiononepithelialmesenchymaltransitionofgastriccancercells AT yongli effectofoverexpressiononepithelialmesenchymaltransitionofgastriccancercells AT bibotan effectofoverexpressiononepithelialmesenchymaltransitionofgastriccancercells AT zhaojiean effectofoverexpressiononepithelialmesenchymaltransitionofgastriccancercells
_version_	1718443959461609472

Effect of overexpression on epithelial–mesenchymal transition of gastric cancer cells

Ejemplares similares