Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells

Abstract The limitations of cisplatin, a standard chemotherapy for lung cancer, have been documented with serious adverse effects and drug resistance. To address the need for novel therapy, this study firstly reveals the potential of peptide from Lentinus squarrosulus (Mont.) as a chemotherapeutic a...

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Autores principales:	Hnin Ei Ei Khine, Gea Abigail Uy Ecoy, Sittiruk Roytrakul, Narumon Phaonakrop, Natapol Pornputtapong, Eakachai Prompetchara, Pithi Chanvorachote, Chatchai Chaotham
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08ba64f8ffb143fcb44a5706131cb743
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spelling	oai:doaj.org-article:08ba64f8ffb143fcb44a5706131cb7432021-12-02T14:03:45ZChemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells10.1038/s41598-021-83606-12045-2322https://doaj.org/article/08ba64f8ffb143fcb44a5706131cb7432021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83606-1https://doaj.org/toc/2045-2322Abstract The limitations of cisplatin, a standard chemotherapy for lung cancer, have been documented with serious adverse effects and drug resistance. To address the need for novel therapy, this study firstly reveals the potential of peptide from Lentinus squarrosulus (Mont.) as a chemotherapeutic adjuvant for cisplatin treatment. The purified peptide from L. squarrosulus aqueous extracts was obtained after eluting with 0.4 M NaCl through FPLC equipped with anion exchange column. Preincubation for 24 h with 5 µg/mL of the peptide at prior to treatment with 5 µM cisplatin significantly diminished %cell viability in various human lung cancer cells but not in human dermal papilla and proximal renal cells. Flow cytometry indicated the augmentation of cisplatin-induced apoptosis in lung cancer cells pretreated with peptide from L. squarrosulus. Preculture with the peptide dramatically inhibited colony formation in lung cancer cells derived after cisplatin treatment. Strong suppression on integrin-mediated survival was evidenced with the diminution of integrins (β1, β3, β5, α5, αV) and down-stream signals (p-FAK/FAK, p-Src/Src, p-Akt/Akt) consequence with alteration of p53, Bax, Blc-2 and Mcl-1 in cisplatin-treated lung cancer cells preincubated with peptide from L. squarrosulus. These results support the development of L. squarrosulus peptide as a novel combined chemotherapy with cisplatin for lung cancer treatment.Hnin Ei Ei KhineGea Abigail Uy EcoySittiruk RoytrakulNarumon PhaonakropNatapol PornputtapongEakachai PrompetcharaPithi ChanvorachoteChatchai ChaothamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hnin Ei Ei Khine Gea Abigail Uy Ecoy Sittiruk Roytrakul Narumon Phaonakrop Natapol Pornputtapong Eakachai Prompetchara Pithi Chanvorachote Chatchai Chaotham Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
description	Abstract The limitations of cisplatin, a standard chemotherapy for lung cancer, have been documented with serious adverse effects and drug resistance. To address the need for novel therapy, this study firstly reveals the potential of peptide from Lentinus squarrosulus (Mont.) as a chemotherapeutic adjuvant for cisplatin treatment. The purified peptide from L. squarrosulus aqueous extracts was obtained after eluting with 0.4 M NaCl through FPLC equipped with anion exchange column. Preincubation for 24 h with 5 µg/mL of the peptide at prior to treatment with 5 µM cisplatin significantly diminished %cell viability in various human lung cancer cells but not in human dermal papilla and proximal renal cells. Flow cytometry indicated the augmentation of cisplatin-induced apoptosis in lung cancer cells pretreated with peptide from L. squarrosulus. Preculture with the peptide dramatically inhibited colony formation in lung cancer cells derived after cisplatin treatment. Strong suppression on integrin-mediated survival was evidenced with the diminution of integrins (β1, β3, β5, α5, αV) and down-stream signals (p-FAK/FAK, p-Src/Src, p-Akt/Akt) consequence with alteration of p53, Bax, Blc-2 and Mcl-1 in cisplatin-treated lung cancer cells preincubated with peptide from L. squarrosulus. These results support the development of L. squarrosulus peptide as a novel combined chemotherapy with cisplatin for lung cancer treatment.
format	article
author	Hnin Ei Ei Khine Gea Abigail Uy Ecoy Sittiruk Roytrakul Narumon Phaonakrop Natapol Pornputtapong Eakachai Prompetchara Pithi Chanvorachote Chatchai Chaotham
author_facet	Hnin Ei Ei Khine Gea Abigail Uy Ecoy Sittiruk Roytrakul Narumon Phaonakrop Natapol Pornputtapong Eakachai Prompetchara Pithi Chanvorachote Chatchai Chaotham
author_sort	Hnin Ei Ei Khine
title	Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
title_short	Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
title_full	Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
title_fullStr	Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
title_full_unstemmed	Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells
title_sort	chemosensitizing activity of peptide from lentinus squarrosulus (mont.) on cisplatin-induced apoptosis in human lung cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/08ba64f8ffb143fcb44a5706131cb743
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Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells

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