Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum

Diatoms are feedstock for the production of sustainable biocommodities, including biofuel. The biochemical characterization of newly isolated or genetically modified strains is seminal to identify the strains that display interesting features for both research and industrial applications. Biochemica...

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Autores principales:	Matteo Scarsini, Adrien Thurotte, Brigitte Veidl, Frederic Amiard, Frederick Niepceron, Myriam Badawi, Fabienne Lagarde, Benoît Schoefs, Justine Marchand
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	FTIR spectroscopy Phaeodactylum tricornutum Bohlin macromolecules quantification algal physiology lipid droplets phytoplankton Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/08bc2743c709483a96f859514c5d2512
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spelling	oai:doaj.org-article:08bc2743c709483a96f859514c5d25122021-11-10T08:24:22ZMetabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum1664-462X10.3389/fpls.2021.756421https://doaj.org/article/08bc2743c709483a96f859514c5d25122021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.756421/fullhttps://doaj.org/toc/1664-462XDiatoms are feedstock for the production of sustainable biocommodities, including biofuel. The biochemical characterization of newly isolated or genetically modified strains is seminal to identify the strains that display interesting features for both research and industrial applications. Biochemical quantification of organic macromolecules cellular quotas are time-consuming methodologies which often require large amount of biological sample. Vibrational spectroscopy is an essential tool applied in several fields of research. A Fourier transform infrared (FTIR) microscopy-based imaging protocol was developed for the simultaneous cellular quota quantification of lipids, carbohydrates, and proteins of the diatom Phaeodactylum tricornutum. The low amount of sample required for the quantification allows the high throughput quantification on small volume cultures. A proof of concept was performed (1) on nitrogen-starved experimental cultures and (2) on three different P. tricornutum wild-type strains. The results are supported by the observation in situ of lipid droplets by confocal and brightfield microscopy. The results show that major differences exist in the regulation of lipid metabolism between ecotypes of P. tricornutum.Matteo ScarsiniAdrien ThurotteAdrien ThurotteBrigitte VeidlFrederic AmiardFrederick NiepceronMyriam BadawiFabienne LagardeBenoît SchoefsJustine MarchandFrontiers Media S.A.articleFTIR spectroscopyPhaeodactylum tricornutum Bohlinmacromolecules quantificationalgal physiologylipid dropletsphytoplanktonPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FTIR spectroscopy Phaeodactylum tricornutum Bohlin macromolecules quantification algal physiology lipid droplets phytoplankton Plant culture SB1-1110
spellingShingle	FTIR spectroscopy Phaeodactylum tricornutum Bohlin macromolecules quantification algal physiology lipid droplets phytoplankton Plant culture SB1-1110 Matteo Scarsini Adrien Thurotte Adrien Thurotte Brigitte Veidl Frederic Amiard Frederick Niepceron Myriam Badawi Fabienne Lagarde Benoît Schoefs Justine Marchand Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
description	Diatoms are feedstock for the production of sustainable biocommodities, including biofuel. The biochemical characterization of newly isolated or genetically modified strains is seminal to identify the strains that display interesting features for both research and industrial applications. Biochemical quantification of organic macromolecules cellular quotas are time-consuming methodologies which often require large amount of biological sample. Vibrational spectroscopy is an essential tool applied in several fields of research. A Fourier transform infrared (FTIR) microscopy-based imaging protocol was developed for the simultaneous cellular quota quantification of lipids, carbohydrates, and proteins of the diatom Phaeodactylum tricornutum. The low amount of sample required for the quantification allows the high throughput quantification on small volume cultures. A proof of concept was performed (1) on nitrogen-starved experimental cultures and (2) on three different P. tricornutum wild-type strains. The results are supported by the observation in situ of lipid droplets by confocal and brightfield microscopy. The results show that major differences exist in the regulation of lipid metabolism between ecotypes of P. tricornutum.
format	article
author	Matteo Scarsini Adrien Thurotte Adrien Thurotte Brigitte Veidl Frederic Amiard Frederick Niepceron Myriam Badawi Fabienne Lagarde Benoît Schoefs Justine Marchand
author_facet	Matteo Scarsini Adrien Thurotte Adrien Thurotte Brigitte Veidl Frederic Amiard Frederick Niepceron Myriam Badawi Fabienne Lagarde Benoît Schoefs Justine Marchand
author_sort	Matteo Scarsini
title	Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
title_short	Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
title_full	Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
title_fullStr	Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
title_full_unstemmed	Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum
title_sort	metabolite quantification by fourier transform infrared spectroscopy in diatoms: proof of concept on phaeodactylum tricornutum
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/08bc2743c709483a96f859514c5d2512
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Metabolite Quantification by Fourier Transform Infrared Spectroscopy in Diatoms: Proof of Concept on Phaeodactylum tricornutum

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