Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break

Polymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insigh...

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Autores principales:	Andrea M. Kaminski, John M. Pryor, Dale A. Ramsden, Thomas A. Kunkel, Lars C. Pedersen, Katarzyna Bebenek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/08bcf0ae3cab403fbdaf9bdc81fef324
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spelling	oai:doaj.org-article:08bcf0ae3cab403fbdaf9bdc81fef3242021-12-02T15:14:56ZStructural snapshots of human DNA polymerase μ engaged on a DNA double-strand break10.1038/s41467-020-18506-52041-1723https://doaj.org/article/08bcf0ae3cab403fbdaf9bdc81fef3242020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18506-5https://doaj.org/toc/2041-1723Polymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insights by allowing the insertion reaction to proceed in crystallo, which enabled them to determine a Polμ structure with incomplete incorporation and the structure of the post-catalytic nicked state.Andrea M. KaminskiJohn M. PryorDale A. RamsdenThomas A. KunkelLars C. PedersenKatarzyna BebenekNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
description	Polymerase μ (Polμ) participates in the repair of DNA double-strand breaks (DSBs) via the nonhomologous end-joining (NHEJ) pathway. Here, the authors determine the crystal structure of a pre-catalytic ternary complex of human Polμ with a bound DSB substrate and they obtain further mechanistic insights by allowing the insertion reaction to proceed in crystallo, which enabled them to determine a Polμ structure with incomplete incorporation and the structure of the post-catalytic nicked state.
format	article
author	Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek
author_facet	Andrea M. Kaminski John M. Pryor Dale A. Ramsden Thomas A. Kunkel Lars C. Pedersen Katarzyna Bebenek
author_sort	Andrea M. Kaminski
title	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_short	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_full	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_fullStr	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_full_unstemmed	Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break
title_sort	structural snapshots of human dna polymerase μ engaged on a dna double-strand break
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/08bcf0ae3cab403fbdaf9bdc81fef324
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Structural snapshots of human DNA polymerase μ engaged on a DNA double-strand break

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