Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats

Abstract This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: the rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), and epithelium-associated (RE) from 1 to 60 d after birth, and manipulate m...

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Autores principales:	Zuo Wang, Chijioke O. Elekwachi, Jinzhen Jiao, Min Wang, Shaoxun Tang, Chuanshe Zhou, Zhiliang Tan, Robert J. Forster
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08beee0580254565bdea87e1befac991
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spelling	oai:doaj.org-article:08beee0580254565bdea87e1befac9912021-12-02T16:07:58ZInvestigation and manipulation of metabolically active methanogen community composition during rumen development in black goats10.1038/s41598-017-00500-52045-2322https://doaj.org/article/08beee0580254565bdea87e1befac9912017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00500-5https://doaj.org/toc/2045-2322Abstract This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: the rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), and epithelium-associated (RE) from 1 to 60 d after birth, and manipulate methanogen community by early weaning on 40 d and supplementing rhubarb from 40 to 60 d in black goats. The RNA-based real-time quantitative PCR and 16S rRNA amplicon sequencing were employed to indicate the metabolically active methanogens. Results showed that active methanogens colonized in RL and RE on 1 d after birth. RP and RE contained the highest and lowest density of methanogens, respectively. Methanobrevibacter, Candidatus Methanomethylophilus, and Methanosphaera were the top three genera. The methanogen communities before weaning differed from those post weaning and the structure of the methanogen community in RE was distinct from those in the other three fractions. The discrepancies in the distribution of methanogens across four fractions, and various fluctuations in abundances among four fractions according to age were observed. The addition of rhubarb significantly (P < 0.05) reduced the abundances of Methanimicrococcus spp. in four fractions on 50 d, but did not change the methanogen community composition on 60 d.Zuo WangChijioke O. ElekwachiJinzhen JiaoMin WangShaoxun TangChuanshe ZhouZhiliang TanRobert J. ForsterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zuo Wang Chijioke O. Elekwachi Jinzhen Jiao Min Wang Shaoxun Tang Chuanshe Zhou Zhiliang Tan Robert J. Forster Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
description	Abstract This study was performed to investigate the initial colonization of metabolically active methanogens and subsequent changes in four fractions: the rumen solid-phase (RS), liquid-phase (RL), protozoa-associated (RP), and epithelium-associated (RE) from 1 to 60 d after birth, and manipulate methanogen community by early weaning on 40 d and supplementing rhubarb from 40 to 60 d in black goats. The RNA-based real-time quantitative PCR and 16S rRNA amplicon sequencing were employed to indicate the metabolically active methanogens. Results showed that active methanogens colonized in RL and RE on 1 d after birth. RP and RE contained the highest and lowest density of methanogens, respectively. Methanobrevibacter, Candidatus Methanomethylophilus, and Methanosphaera were the top three genera. The methanogen communities before weaning differed from those post weaning and the structure of the methanogen community in RE was distinct from those in the other three fractions. The discrepancies in the distribution of methanogens across four fractions, and various fluctuations in abundances among four fractions according to age were observed. The addition of rhubarb significantly (P < 0.05) reduced the abundances of Methanimicrococcus spp. in four fractions on 50 d, but did not change the methanogen community composition on 60 d.
format	article
author	Zuo Wang Chijioke O. Elekwachi Jinzhen Jiao Min Wang Shaoxun Tang Chuanshe Zhou Zhiliang Tan Robert J. Forster
author_facet	Zuo Wang Chijioke O. Elekwachi Jinzhen Jiao Min Wang Shaoxun Tang Chuanshe Zhou Zhiliang Tan Robert J. Forster
author_sort	Zuo Wang
title	Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
title_short	Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
title_full	Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
title_fullStr	Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
title_full_unstemmed	Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
title_sort	investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/08beee0580254565bdea87e1befac991
work_keys_str_mv	AT zuowang investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT chijiokeoelekwachi investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT jinzhenjiao investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT minwang investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT shaoxuntang investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT chuanshezhou investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT zhiliangtan investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats AT robertjforster investigationandmanipulationofmetabolicallyactivemethanogencommunitycompositionduringrumendevelopmentinblackgoats
_version_	1718384637017849856

Investigation and manipulation of metabolically active methanogen community composition during rumen development in black goats

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