Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury

Abstract This study examined the efficacy of end-ischemic hypothermic oxygenated machine perfusion preservation (HOPE) using an originally developed machine perfusion system for split-liver transplantation. Porcine split-liver grafts were created via 75% liver resection after 10 min of warm ischemia...

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Autores principales: Daisuke Ishii, Naoto Matsuno, Mikako Gochi, Hiroyoshi Iwata, Tatsuya Shonaka, Yuji Nishikawa, Hiromichi Obara, Hideki Yokoo, Hiroyuki Furukawa
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/08c00880409347a58bd080afcbdbb56b
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spelling oai:doaj.org-article:08c00880409347a58bd080afcbdbb56b2021-11-21T12:19:21ZBeneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury10.1038/s41598-021-01467-02045-2322https://doaj.org/article/08c00880409347a58bd080afcbdbb56b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01467-0https://doaj.org/toc/2045-2322Abstract This study examined the efficacy of end-ischemic hypothermic oxygenated machine perfusion preservation (HOPE) using an originally developed machine perfusion system for split-liver transplantation. Porcine split-liver grafts were created via 75% liver resection after 10 min of warm ischemia. In Group 1, grafts were preserved by simple cold storage (CS) for 8 h (CS group; n = 4). In Group 2, grafts were preserved by simple CS for 6 h and end-ischemic HOPE for 2 h (HOPE group; n = 5). All grafts were evaluated using an isolated ex vivo reperfusion model with autologous blood for 2 h. Biochemical markers (aspartate aminotransferase and lactate dehydrogenase levels) were significantly better immediately after reperfusion in the HOPE group than in the CS group. Furthermore, the HOPE group had a better histological score. The levels of inflammatory cytokines (tumor necrosis factor-α, interferon-γ, interleukin-1β, and interleukin-10) were significantly lower after reperfusion in the HOPE group. Therefore, we concluded that end-ischemic HOPE for split-liver transplantation can aid in recovering the graft function and reducing ischemia–reperfusion injury. HOPE, using our originally developed machine perfusion system, is safe and can improve graft function while attenuating liver injury due to preservation.Daisuke IshiiNaoto MatsunoMikako GochiHiroyoshi IwataTatsuya ShonakaYuji NishikawaHiromichi ObaraHideki YokooHiroyuki FurukawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Daisuke Ishii
Naoto Matsuno
Mikako Gochi
Hiroyoshi Iwata
Tatsuya Shonaka
Yuji Nishikawa
Hiromichi Obara
Hideki Yokoo
Hiroyuki Furukawa
Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
description Abstract This study examined the efficacy of end-ischemic hypothermic oxygenated machine perfusion preservation (HOPE) using an originally developed machine perfusion system for split-liver transplantation. Porcine split-liver grafts were created via 75% liver resection after 10 min of warm ischemia. In Group 1, grafts were preserved by simple cold storage (CS) for 8 h (CS group; n = 4). In Group 2, grafts were preserved by simple CS for 6 h and end-ischemic HOPE for 2 h (HOPE group; n = 5). All grafts were evaluated using an isolated ex vivo reperfusion model with autologous blood for 2 h. Biochemical markers (aspartate aminotransferase and lactate dehydrogenase levels) were significantly better immediately after reperfusion in the HOPE group than in the CS group. Furthermore, the HOPE group had a better histological score. The levels of inflammatory cytokines (tumor necrosis factor-α, interferon-γ, interleukin-1β, and interleukin-10) were significantly lower after reperfusion in the HOPE group. Therefore, we concluded that end-ischemic HOPE for split-liver transplantation can aid in recovering the graft function and reducing ischemia–reperfusion injury. HOPE, using our originally developed machine perfusion system, is safe and can improve graft function while attenuating liver injury due to preservation.
format article
author Daisuke Ishii
Naoto Matsuno
Mikako Gochi
Hiroyoshi Iwata
Tatsuya Shonaka
Yuji Nishikawa
Hiromichi Obara
Hideki Yokoo
Hiroyuki Furukawa
author_facet Daisuke Ishii
Naoto Matsuno
Mikako Gochi
Hiroyoshi Iwata
Tatsuya Shonaka
Yuji Nishikawa
Hiromichi Obara
Hideki Yokoo
Hiroyuki Furukawa
author_sort Daisuke Ishii
title Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
title_short Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
title_full Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
title_fullStr Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
title_full_unstemmed Beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
title_sort beneficial effects of end-ischemic oxygenated machine perfusion preservation for split-liver transplantation in recovering graft function and reducing ischemia–reperfusion injury
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/08c00880409347a58bd080afcbdbb56b
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