mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells

Guoxi Jin,1,2 Qiong Wang,2 Xiaoyan Pei,2 Xiaoli Li,2 Xiaolei Hu,2 Erqin Xu,3 Minglong Li11Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, People’s Republic of China; 2Department of Endocrinology, The First Affili...

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Autores principales:	Jin G, Wang Q, Pei X, Li X, Hu X, Xu E, Li M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Diabetes High glucose Metabolic memory Messenger RNA (mRNA) RNA-sequencing Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/08c1504ad1ab46638da5e3dd9bd3cbcd
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spelling	oai:doaj.org-article:08c1504ad1ab46638da5e3dd9bd3cbcd2021-12-02T06:27:20ZmRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells1178-7007https://doaj.org/article/08c1504ad1ab46638da5e3dd9bd3cbcd2019-07-01T00:00:00Zhttps://www.dovepress.com/mrnas-expression-profiles-of-high-glucose-induced-memory-in-human-umbi-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Guoxi Jin,1,2 Qiong Wang,2 Xiaoyan Pei,2 Xiaoli Li,2 Xiaolei Hu,2 Erqin Xu,3 Minglong Li11Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, People’s Republic of China; 2Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, People’s Republic of China; 3Room of Physical Diagnostics, Clinical College of Medicine, Bengbu Medical College, Bengbu, Anhui 233030, People’s Republic of ChinaPurpose: A long-term “memory” of hyperglycemic stress, even when glycemia is normalized, has been previously reported in endothelial cells. However, the molecular mechanism of “metabolic memory” (MM) remains unknown. In this report, we sought to screen at the whole transcriptome level the genes that participate in MM.Methods: In the present research, RNA sequencing was used to determine the protein-coding mRNA expression profiles of human umbilical vein endothelial cells (HUVECs) under normal-glucose concentration (LG), high-glucose concentration (HG), and MM. A series of bioinformatic analyses was performed. HG-induced MM-involved up-regulated genes (up-HGMMGs) and HG-induced MM-involved down-regulated genes (down-HGMMGs) were identified. Afterward, based on up-HGMMGs and down-HGMMGs, the biological functions and signaling pathways were analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, several of the identified genes were validated by RT-qPCR.Results: A total of 726 HGMMGs were identified, including 210 down- and 516 up-HGMMGs, which were enriched in the cell cycle (hsa04110), oocyte meiosis (hsa04114), p53 signaling pathway (hsa04115), and oxidative phosphorylation (hsa00190), among others. The protein–protein-interaction (PPI) network consisted of 462 nodes and 2656 connections, and four main modules were identified by MCODE. The cell cycle (hsa04110), oocyte meiosis (hsa04114), p53 signaling pathway (hsa04115), and oxidative phosphorylation (hsa00190), among others, could be potential therapeutic targets of HG-induced MM in endothelial cells. The real-time PCR results validated the RNA-seq data.Conclusion: This study identified crucial mRNAs related to MM-persistent injury in endothelial cells even after switching the cells from high- glucose to normal glucose levels. Further research focusing on these mRNA may unravel new ways to modify MM in diabetes.Keywords: diabetes, high glucose, metabolic memory, messenger RNA, RNA-sequencingJin GWang QPei XLi XHu XXu ELi MDove Medical PressarticleDiabetesHigh glucoseMetabolic memoryMessenger RNA (mRNA)RNA-sequencingSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 1249-1261 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diabetes High glucose Metabolic memory Messenger RNA (mRNA) RNA-sequencing Specialties of internal medicine RC581-951
spellingShingle	Diabetes High glucose Metabolic memory Messenger RNA (mRNA) RNA-sequencing Specialties of internal medicine RC581-951 Jin G Wang Q Pei X Li X Hu X Xu E Li M mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
description	Guoxi Jin,1,2 Qiong Wang,2 Xiaoyan Pei,2 Xiaoli Li,2 Xiaolei Hu,2 Erqin Xu,3 Minglong Li11Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, People’s Republic of China; 2Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, People’s Republic of China; 3Room of Physical Diagnostics, Clinical College of Medicine, Bengbu Medical College, Bengbu, Anhui 233030, People’s Republic of ChinaPurpose: A long-term “memory” of hyperglycemic stress, even when glycemia is normalized, has been previously reported in endothelial cells. However, the molecular mechanism of “metabolic memory” (MM) remains unknown. In this report, we sought to screen at the whole transcriptome level the genes that participate in MM.Methods: In the present research, RNA sequencing was used to determine the protein-coding mRNA expression profiles of human umbilical vein endothelial cells (HUVECs) under normal-glucose concentration (LG), high-glucose concentration (HG), and MM. A series of bioinformatic analyses was performed. HG-induced MM-involved up-regulated genes (up-HGMMGs) and HG-induced MM-involved down-regulated genes (down-HGMMGs) were identified. Afterward, based on up-HGMMGs and down-HGMMGs, the biological functions and signaling pathways were analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). In addition, several of the identified genes were validated by RT-qPCR.Results: A total of 726 HGMMGs were identified, including 210 down- and 516 up-HGMMGs, which were enriched in the cell cycle (hsa04110), oocyte meiosis (hsa04114), p53 signaling pathway (hsa04115), and oxidative phosphorylation (hsa00190), among others. The protein–protein-interaction (PPI) network consisted of 462 nodes and 2656 connections, and four main modules were identified by MCODE. The cell cycle (hsa04110), oocyte meiosis (hsa04114), p53 signaling pathway (hsa04115), and oxidative phosphorylation (hsa00190), among others, could be potential therapeutic targets of HG-induced MM in endothelial cells. The real-time PCR results validated the RNA-seq data.Conclusion: This study identified crucial mRNAs related to MM-persistent injury in endothelial cells even after switching the cells from high- glucose to normal glucose levels. Further research focusing on these mRNA may unravel new ways to modify MM in diabetes.Keywords: diabetes, high glucose, metabolic memory, messenger RNA, RNA-sequencing
format	article
author	Jin G Wang Q Pei X Li X Hu X Xu E Li M
author_facet	Jin G Wang Q Pei X Li X Hu X Xu E Li M
author_sort	Jin G
title	mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
title_short	mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
title_full	mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
title_fullStr	mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
title_full_unstemmed	mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
title_sort	mrnas expression profiles of high glucose-induced memory in human umbilical vein endothelial cells
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/08c1504ad1ab46638da5e3dd9bd3cbcd
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mRNAs expression profiles of high glucose-induced memory in human umbilical vein endothelial cells

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