Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos

Abstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the uti...

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Autores principales:	Ian G. Brennan, Aaron M. Bauer, Ngo Van Tri, Yun-yu Wang, Wen-zhi Wang, Ya-Ping Zhang, Robert W. Murphy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08c348547e134350993c14871d9dce26
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id	oai:doaj.org-article:08c348547e134350993c14871d9dce26
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spelling	oai:doaj.org-article:08c348547e134350993c14871d9dce262021-12-02T11:52:21ZBarcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos10.1038/s41598-017-05261-92045-2322https://doaj.org/article/08c348547e134350993c14871d9dce262017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05261-9https://doaj.org/toc/2045-2322Abstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the utility of barcoding data for the taxonomic group of interest. Here, our primary aim is to determine the phylogenetic value of DNA barcoding (mitochondrial locus COI) within the gecko genus Cyrtodactylus. With >40 new species described since last systematic investigation, Cyrtodactylus represents one of the most diverse extant squamate genera, and their contemporary distribution spans the Indian subcontinent, eastward through Indochina, and into AustraloPapua. The complex biogeographic history of this group, and morphology-only designation of many species have complicated our phylogenetic understanding of Cyrtodactylus. To highlight the need for continued inclusive molecular assessment, we use Vietnamese Cyrtodactylus as a case study showing the geopolitically paraphyletic nature of their history. We compare COI to the legacy marker ND2, and discuss the value of COI as an interspecific marker, as well as its shortcomings at deeper evolutionary scales. We draw attention back to the Cold Code as a subsidized method for incorporating molecular methods into species descriptions in the effort to maintain accurate phylogenies.Ian G. BrennanAaron M. BauerNgo Van TriYun-yu WangWen-zhi WangYa-Ping ZhangRobert W. MurphyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
description	Abstract Over the past decade, DNA barcoding has become a staple of low-cost molecular systematic investigations. The availability of universal primers and subsidized sequencing projects (PolarBOL, SharkBOL, SpongeBOL) have driven this popularity, often without appropriate investigation into the utility of barcoding data for the taxonomic group of interest. Here, our primary aim is to determine the phylogenetic value of DNA barcoding (mitochondrial locus COI) within the gecko genus Cyrtodactylus. With >40 new species described since last systematic investigation, Cyrtodactylus represents one of the most diverse extant squamate genera, and their contemporary distribution spans the Indian subcontinent, eastward through Indochina, and into AustraloPapua. The complex biogeographic history of this group, and morphology-only designation of many species have complicated our phylogenetic understanding of Cyrtodactylus. To highlight the need for continued inclusive molecular assessment, we use Vietnamese Cyrtodactylus as a case study showing the geopolitically paraphyletic nature of their history. We compare COI to the legacy marker ND2, and discuss the value of COI as an interspecific marker, as well as its shortcomings at deeper evolutionary scales. We draw attention back to the Cold Code as a subsidized method for incorporating molecular methods into species descriptions in the effort to maintain accurate phylogenies.
format	article
author	Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy
author_facet	Ian G. Brennan Aaron M. Bauer Ngo Van Tri Yun-yu Wang Wen-zhi Wang Ya-Ping Zhang Robert W. Murphy
author_sort	Ian G. Brennan
title	Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
title_short	Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
title_full	Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
title_fullStr	Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
title_full_unstemmed	Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos
title_sort	barcoding utility in a mega-diverse, cross-continental genus: keeping pace with cyrtodactylus geckos
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/08c348547e134350993c14871d9dce26
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Barcoding utility in a mega-diverse, cross-continental genus: keeping pace with Cyrtodactylus geckos

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