A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.

Adherent-invasive Escherichia coli (AIEC) has been linked with the onset and perpetuation of inflammatory bowel diseases. The AIEC strain LF82 was originally isolated from an ileal biopsy from a patient with Crohn's disease. The pathogenesis of LF82 results from its abnormal adherence to and su...

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Autores principales:	Karina T Simonsen, Gorm Nielsen, Janni Vester Bjerrum, Thomas Kruse, Birgitte H Kallipolitis, Jakob Møller-Jensen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08c3fa98e7ea4dc1ab8ccaf639d2f550
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spelling	oai:doaj.org-article:08c3fa98e7ea4dc1ab8ccaf639d2f5502021-11-18T06:59:49ZA role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.1932-620310.1371/journal.pone.0016387https://doaj.org/article/08c3fa98e7ea4dc1ab8ccaf639d2f5502011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21298102/?tool=EBIhttps://doaj.org/toc/1932-6203Adherent-invasive Escherichia coli (AIEC) has been linked with the onset and perpetuation of inflammatory bowel diseases. The AIEC strain LF82 was originally isolated from an ileal biopsy from a patient with Crohn's disease. The pathogenesis of LF82 results from its abnormal adherence to and subsequent invasion of the intestinal epithelium coupled with its ability to survive phagocytosis by macrophages once it has crossed the intestinal barrier. To gain further insight into AIEC pathogenesis we employed the nematode Caenorhabditis elegans as an in vivo infection model. We demonstrate that AIEC strain LF82 forms a persistent infection in C. elegans, thereby reducing the host lifespan significantly. This host killing phenotype was associated with massive bacterial colonization of the nematode intestine and damage to the intestinal epithelial surface. C. elegans killing was independent of known LF82 virulence determinants but was abolished by deletion of the LF82 hfq gene, which encodes an RNA chaperone involved in mediating posttranscriptional gene regulation by small non-coding RNAs. This finding reveals that important aspects of LF82 pathogenesis are controlled at the posttranscriptional level by riboregulation. The role of Hfq in LF82 virulence was independent of its function in regulating RpoS and RpoE activity. Further, LF82Δhfq mutants were non-motile, impaired in cell invasion and highly sensitive to various chemical stress conditions, reinforcing the multifaceted function of Hfq in mediating bacterial adaptation. This study highlights the usefulness of simple non-mammalian infection systems for the identification and analysis of bacterial virulence factors.Karina T SimonsenGorm NielsenJanni Vester BjerrumThomas KruseBirgitte H KallipolitisJakob Møller-JensenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16387 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karina T Simonsen Gorm Nielsen Janni Vester Bjerrum Thomas Kruse Birgitte H Kallipolitis Jakob Møller-Jensen A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
description	Adherent-invasive Escherichia coli (AIEC) has been linked with the onset and perpetuation of inflammatory bowel diseases. The AIEC strain LF82 was originally isolated from an ileal biopsy from a patient with Crohn's disease. The pathogenesis of LF82 results from its abnormal adherence to and subsequent invasion of the intestinal epithelium coupled with its ability to survive phagocytosis by macrophages once it has crossed the intestinal barrier. To gain further insight into AIEC pathogenesis we employed the nematode Caenorhabditis elegans as an in vivo infection model. We demonstrate that AIEC strain LF82 forms a persistent infection in C. elegans, thereby reducing the host lifespan significantly. This host killing phenotype was associated with massive bacterial colonization of the nematode intestine and damage to the intestinal epithelial surface. C. elegans killing was independent of known LF82 virulence determinants but was abolished by deletion of the LF82 hfq gene, which encodes an RNA chaperone involved in mediating posttranscriptional gene regulation by small non-coding RNAs. This finding reveals that important aspects of LF82 pathogenesis are controlled at the posttranscriptional level by riboregulation. The role of Hfq in LF82 virulence was independent of its function in regulating RpoS and RpoE activity. Further, LF82Δhfq mutants were non-motile, impaired in cell invasion and highly sensitive to various chemical stress conditions, reinforcing the multifaceted function of Hfq in mediating bacterial adaptation. This study highlights the usefulness of simple non-mammalian infection systems for the identification and analysis of bacterial virulence factors.
format	article
author	Karina T Simonsen Gorm Nielsen Janni Vester Bjerrum Thomas Kruse Birgitte H Kallipolitis Jakob Møller-Jensen
author_facet	Karina T Simonsen Gorm Nielsen Janni Vester Bjerrum Thomas Kruse Birgitte H Kallipolitis Jakob Møller-Jensen
author_sort	Karina T Simonsen
title	A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
title_short	A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
title_full	A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
title_fullStr	A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
title_full_unstemmed	A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.
title_sort	role for the rna chaperone hfq in controlling adherent-invasive escherichia coli colonization and virulence.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/08c3fa98e7ea4dc1ab8ccaf639d2f550
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A role for the RNA chaperone Hfq in controlling adherent-invasive Escherichia coli colonization and virulence.

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