Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.

A main determinant of prolonged Trypanosoma brucei infection and transmission and success of the parasite is the interplay between host acquired immunity and antigenic variation of the parasite variant surface glycoprotein (VSG) coat. About 0.1% of trypanosome divisions produce a switch to a differe...

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Autores principales:	James P J Hall, Huanhuan Wang, J David Barry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/08cd7d39b38b425e8812445a0f409546
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spelling	oai:doaj.org-article:08cd7d39b38b425e8812445a0f4095462021-11-18T06:05:23ZMosaic VSGs and the scale of Trypanosoma brucei antigenic variation.1553-73661553-737410.1371/journal.ppat.1003502https://doaj.org/article/08cd7d39b38b425e8812445a0f4095462013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23853603/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374A main determinant of prolonged Trypanosoma brucei infection and transmission and success of the parasite is the interplay between host acquired immunity and antigenic variation of the parasite variant surface glycoprotein (VSG) coat. About 0.1% of trypanosome divisions produce a switch to a different VSG through differential expression of an archive of hundreds of silent VSG genes and pseudogenes, but the patterns and extent of the trypanosome diversity phenotype, particularly in chronic infection, are unclear. We applied longitudinal VSG cDNA sequencing to estimate variant richness and test whether pseudogenes contribute to antigenic variation. We show that individual growth peaks can contain at least 15 distinct variants, are estimated computationally to comprise many more, and that antigenically distinct 'mosaic' VSGs arise from segmental gene conversion between donor VSG genes or pseudogenes. The potential for trypanosome antigenic variation is probably much greater than VSG archive size; mosaic VSGs are core to antigenic variation and chronic infection.James P J HallHuanhuan WangJ David BarryPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 7, p e1003502 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 James P J Hall Huanhuan Wang J David Barry Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
description	A main determinant of prolonged Trypanosoma brucei infection and transmission and success of the parasite is the interplay between host acquired immunity and antigenic variation of the parasite variant surface glycoprotein (VSG) coat. About 0.1% of trypanosome divisions produce a switch to a different VSG through differential expression of an archive of hundreds of silent VSG genes and pseudogenes, but the patterns and extent of the trypanosome diversity phenotype, particularly in chronic infection, are unclear. We applied longitudinal VSG cDNA sequencing to estimate variant richness and test whether pseudogenes contribute to antigenic variation. We show that individual growth peaks can contain at least 15 distinct variants, are estimated computationally to comprise many more, and that antigenically distinct 'mosaic' VSGs arise from segmental gene conversion between donor VSG genes or pseudogenes. The potential for trypanosome antigenic variation is probably much greater than VSG archive size; mosaic VSGs are core to antigenic variation and chronic infection.
format	article
author	James P J Hall Huanhuan Wang J David Barry
author_facet	James P J Hall Huanhuan Wang J David Barry
author_sort	James P J Hall
title	Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
title_short	Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
title_full	Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
title_fullStr	Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
title_full_unstemmed	Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.
title_sort	mosaic vsgs and the scale of trypanosoma brucei antigenic variation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/08cd7d39b38b425e8812445a0f409546
work_keys_str_mv	AT jamespjhall mosaicvsgsandthescaleoftrypanosomabruceiantigenicvariation AT huanhuanwang mosaicvsgsandthescaleoftrypanosomabruceiantigenicvariation AT jdavidbarry mosaicvsgsandthescaleoftrypanosomabruceiantigenicvariation
_version_	1718424582145179648

Mosaic VSGs and the scale of Trypanosoma brucei antigenic variation.

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