Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities

Background and Objectives: Actinobacteria efficiently can produce different nanoparticles with various biological properties due to their ability to produce secondary metabolites. The present study aimed to examine the isolation and screening of gold nanoparticles via producing actinobacteria from t...

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Autores principales:	Faezeh kabiri, Seyyed Soheil Aghaei, Ahmad Ali Pourbabaee, Mohammad Soleimani, Tahereh Komeili Movahhed
Formato:	article
Lenguaje:	FA
Publicado:	Qom University of Medical Sciences 2021
Materias:	actinobacteria gold nanoparticles green synthesizes antibacteria activity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/08cdc90785674060a75335389ac89b9f
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spelling	oai:doaj.org-article:08cdc90785674060a75335389ac89b9f2021-12-05T11:40:11ZManganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities1735-77992008-1375https://doaj.org/article/08cdc90785674060a75335389ac89b9f2021-05-01T00:00:00Zhttp://journal.muq.ac.ir/article-1-3158-en.htmlhttps://doaj.org/toc/1735-7799https://doaj.org/toc/2008-1375Background and Objectives: Actinobacteria efficiently can produce different nanoparticles with various biological properties due to their ability to produce secondary metabolites. The present study aimed to examine the isolation and screening of gold nanoparticles via producing actinobacteria from the soil. We also studied their antibacterial activities. Methods: In this study, after the isolation of actinobacteria, the accumulation of gold nanoparticles was investigated. This aim was achieved by changing the color of the reaction medium and its adsorption by UV-visible spectrophotometry. Then, for conducting confirmatory tests, ultraviolet and visible absorption spectroscopy, high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and dynamic light scattering analysis was used. Phenotypic and molecular methods were applied to study the nanoparticle-producing actinobacterium isolate. The antibacterial activity of synthesized gold was explored on pathogenic bacteria. Results: Among the examined 35 Actinobacteria strains, one strain was biosynthesized nanoparticles; it indicated the maximum absorption at 537 nm, i.e., the characteristic of gold nanoparticles. Dynamic light scattering analysis revealed an average size of 44.4 nm. The minimum inhibitory concentration of gold nanoparticles against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus was 5.8μg/mL, 11.7μg/mL, and 11.7μg/mL, respectively. Molecular analysis data revealed that the Actinobacteria isolate was Amycolatopsis sp. KMN. The isolate Amycolatopsis sp. strain KMN presented 99.82% homology to Amycolatopsis methanolica239. Conclusion: Overall, according to the obtained results, nanoparticles biosynthesized using Actinobacter cell extract suggested different properties, making them a suitable candidate for further research concerning nanomedicines.Faezeh kabiriSeyyed Soheil AghaeiAhmad Ali PourbabaeeMohammad SoleimaniTahereh Komeili MovahhedQom University of Medical Sciencesarticleactinobacteriagoldnanoparticlesgreen synthesizesantibacteria activityMedicine (General)R5-920FAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 15, Iss 3, Pp 178-187 (2021)
institution	DOAJ
collection	DOAJ
language	FA
topic	actinobacteria gold nanoparticles green synthesizes antibacteria activity Medicine (General) R5-920
spellingShingle	actinobacteria gold nanoparticles green synthesizes antibacteria activity Medicine (General) R5-920 Faezeh kabiri Seyyed Soheil Aghaei Ahmad Ali Pourbabaee Mohammad Soleimani Tahereh Komeili Movahhed Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
description	Background and Objectives: Actinobacteria efficiently can produce different nanoparticles with various biological properties due to their ability to produce secondary metabolites. The present study aimed to examine the isolation and screening of gold nanoparticles via producing actinobacteria from the soil. We also studied their antibacterial activities. Methods: In this study, after the isolation of actinobacteria, the accumulation of gold nanoparticles was investigated. This aim was achieved by changing the color of the reaction medium and its adsorption by UV-visible spectrophotometry. Then, for conducting confirmatory tests, ultraviolet and visible absorption spectroscopy, high-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and dynamic light scattering analysis was used. Phenotypic and molecular methods were applied to study the nanoparticle-producing actinobacterium isolate. The antibacterial activity of synthesized gold was explored on pathogenic bacteria. Results: Among the examined 35 Actinobacteria strains, one strain was biosynthesized nanoparticles; it indicated the maximum absorption at 537 nm, i.e., the characteristic of gold nanoparticles. Dynamic light scattering analysis revealed an average size of 44.4 nm. The minimum inhibitory concentration of gold nanoparticles against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus was 5.8μg/mL, 11.7μg/mL, and 11.7μg/mL, respectively. Molecular analysis data revealed that the Actinobacteria isolate was Amycolatopsis sp. KMN. The isolate Amycolatopsis sp. strain KMN presented 99.82% homology to Amycolatopsis methanolica239. Conclusion: Overall, according to the obtained results, nanoparticles biosynthesized using Actinobacter cell extract suggested different properties, making them a suitable candidate for further research concerning nanomedicines.
format	article
author	Faezeh kabiri Seyyed Soheil Aghaei Ahmad Ali Pourbabaee Mohammad Soleimani Tahereh Komeili Movahhed
author_facet	Faezeh kabiri Seyyed Soheil Aghaei Ahmad Ali Pourbabaee Mohammad Soleimani Tahereh Komeili Movahhed
author_sort	Faezeh kabiri
title	Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
title_short	Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
title_full	Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
title_fullStr	Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
title_full_unstemmed	Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities
title_sort	manganese mine actinobacterial mediated gold nanoparticles synthesis and their antibacterial activities
publisher	Qom University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/08cdc90785674060a75335389ac89b9f
work_keys_str_mv	AT faezehkabiri manganesemineactinobacterialmediatedgoldnanoparticlessynthesisandtheirantibacterialactivities AT seyyedsoheilaghaei manganesemineactinobacterialmediatedgoldnanoparticlessynthesisandtheirantibacterialactivities AT ahmadalipourbabaee manganesemineactinobacterialmediatedgoldnanoparticlessynthesisandtheirantibacterialactivities AT mohammadsoleimani manganesemineactinobacterialmediatedgoldnanoparticlessynthesisandtheirantibacterialactivities AT taherehkomeilimovahhed manganesemineactinobacterialmediatedgoldnanoparticlessynthesisandtheirantibacterialactivities
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Manganese Mine Actinobacterial Mediated Gold Nanoparticles Synthesis and Their Antibacterial Activities

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