A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.

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Autores principales: Sarah A. Nordeen, Kasper R. Andersen, Kevin E. Knockenhauer, Jessica R. Ingram, Hidde L. Ploegh, Thomas U. Schwartz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/08cecb6773264203972c596074acd9d4
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spelling oai:doaj.org-article:08cecb6773264203972c596074acd9d42021-12-02T17:06:31ZA nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure10.1038/s41467-020-19884-62041-1723https://doaj.org/article/08cecb6773264203972c596074acd9d42020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19884-6https://doaj.org/toc/2041-1723Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.Sarah A. NordeenKasper R. AndersenKevin E. KnockenhauerJessica R. IngramHidde L. PloeghThomas U. SchwartzNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
description Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.
format article
author Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
author_facet Sarah A. Nordeen
Kasper R. Andersen
Kevin E. Knockenhauer
Jessica R. Ingram
Hidde L. Ploegh
Thomas U. Schwartz
author_sort Sarah A. Nordeen
title A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_short A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_full A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_fullStr A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_full_unstemmed A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
title_sort nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/08cecb6773264203972c596074acd9d4
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