A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure
Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.
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Main Authors: | , , , , , |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2020
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Subjects: | |
Online Access: | https://doaj.org/article/08cecb6773264203972c596074acd9d4 |
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