A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure

Characterizing the assembly of the nuclear pore complex (NPC) remains challenging. Here, the authors develop a set of nanobodies that recognize seven constituent nucleoporins, study their binding characteristics, and apply them to probe accessible and obstructed NPC surfaces in yeast.

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Autores principales: Sarah A. Nordeen, Kasper R. Andersen, Kevin E. Knockenhauer, Jessica R. Ingram, Hidde L. Ploegh, Thomas U. Schwartz
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
Materias:
Science
Q
Acceso en línea:https://doaj.org/article/08cecb6773264203972c596074acd9d4
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