Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis

Synchronized uterine receptivity with the time of implantation is crucial for pregnancy continuity. Vitamin D (VD) deficiency has been linked to the failure of implantation. Therefore, we tested the link between the Homeobox transcription factor-10/immunophilin FK506-binding protein 52 (HOXA-10/FKBP...

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Autores principales:	Hend Ashour, Sara Mahmoud Gamal, Nermeen Bakr Sadek, Laila Ahmed Rashed, Rania Elsayed Hussein, Samaa Samir Kamar, Hayam Ateyya, Marwa Nagi Mehesen, Asmaa Mohammed ShamsEldeen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	vitamin D contraction progesterone receptor decidualization HOXA-10/FKBP52 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/08d2a0c0e5cf4f2185cbc5b3e5188ea2
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spelling	oai:doaj.org-article:08d2a0c0e5cf4f2185cbc5b3e5188ea22021-12-01T00:21:44ZVitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis1664-042X10.3389/fphys.2021.744548https://doaj.org/article/08d2a0c0e5cf4f2185cbc5b3e5188ea22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.744548/fullhttps://doaj.org/toc/1664-042XSynchronized uterine receptivity with the time of implantation is crucial for pregnancy continuity. Vitamin D (VD) deficiency has been linked to the failure of implantation. Therefore, we tested the link between the Homeobox transcription factor-10/immunophilin FK506-binding protein 52 (HOXA-10/FKBP52) axis and the uterine receptivity in VD-deficient rats. The effect of VD supplementation at different doses was also investigated. Forty-eight pregnant rats were divided into six groups (eight/group); normal control rats fed with standard chow (control), control rats supplemented with VD (equivalent dose of 400 IU/day) (control-D400). VD-deficient group (DEF) and the three VD deficiency groups with VD supplementation were equivalent to 400, 4,000, and 10,000 IU/day (DEF-D400, DEF-D4000, and DEF-D10000, respectively). The expression levels of HOXA-10/FKBP52, progesterone level, and histological evaluation of decidualization using osteopontin (OSN) and progesterone receptor (PGR) were estimated. An assessment of the uterine contractility was conducted for all rats. This study showed the downregulation of HOXA-10/FKBP52 together with increased amplitude and frequency of the uterine contractility in the DEF group compared to control. VD dose-dependent supplementation restored progesterone/receptor competency, upregulated the expressional response of HOXA-10 and its downstream FKBP52, and improved uterine receptivity and endometrial decidualization at the time of implantation that was documented by increased area% of OSN and the number of implantation beads.Hend AshourHend AshourSara Mahmoud GamalNermeen Bakr SadekLaila Ahmed RashedRania Elsayed HusseinSamaa Samir KamarSamaa Samir KamarHayam AteyyaHayam AteyyaMarwa Nagi MehesenAsmaa Mohammed ShamsEldeenFrontiers Media S.A.articlevitamin Dcontractionprogesterone receptordecidualizationHOXA-10/FKBP52PhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vitamin D contraction progesterone receptor decidualization HOXA-10/FKBP52 Physiology QP1-981
spellingShingle	vitamin D contraction progesterone receptor decidualization HOXA-10/FKBP52 Physiology QP1-981 Hend Ashour Hend Ashour Sara Mahmoud Gamal Nermeen Bakr Sadek Laila Ahmed Rashed Rania Elsayed Hussein Samaa Samir Kamar Samaa Samir Kamar Hayam Ateyya Hayam Ateyya Marwa Nagi Mehesen Asmaa Mohammed ShamsEldeen Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
description	Synchronized uterine receptivity with the time of implantation is crucial for pregnancy continuity. Vitamin D (VD) deficiency has been linked to the failure of implantation. Therefore, we tested the link between the Homeobox transcription factor-10/immunophilin FK506-binding protein 52 (HOXA-10/FKBP52) axis and the uterine receptivity in VD-deficient rats. The effect of VD supplementation at different doses was also investigated. Forty-eight pregnant rats were divided into six groups (eight/group); normal control rats fed with standard chow (control), control rats supplemented with VD (equivalent dose of 400 IU/day) (control-D400). VD-deficient group (DEF) and the three VD deficiency groups with VD supplementation were equivalent to 400, 4,000, and 10,000 IU/day (DEF-D400, DEF-D4000, and DEF-D10000, respectively). The expression levels of HOXA-10/FKBP52, progesterone level, and histological evaluation of decidualization using osteopontin (OSN) and progesterone receptor (PGR) were estimated. An assessment of the uterine contractility was conducted for all rats. This study showed the downregulation of HOXA-10/FKBP52 together with increased amplitude and frequency of the uterine contractility in the DEF group compared to control. VD dose-dependent supplementation restored progesterone/receptor competency, upregulated the expressional response of HOXA-10 and its downstream FKBP52, and improved uterine receptivity and endometrial decidualization at the time of implantation that was documented by increased area% of OSN and the number of implantation beads.
format	article
author	Hend Ashour Hend Ashour Sara Mahmoud Gamal Nermeen Bakr Sadek Laila Ahmed Rashed Rania Elsayed Hussein Samaa Samir Kamar Samaa Samir Kamar Hayam Ateyya Hayam Ateyya Marwa Nagi Mehesen Asmaa Mohammed ShamsEldeen
author_facet	Hend Ashour Hend Ashour Sara Mahmoud Gamal Nermeen Bakr Sadek Laila Ahmed Rashed Rania Elsayed Hussein Samaa Samir Kamar Samaa Samir Kamar Hayam Ateyya Hayam Ateyya Marwa Nagi Mehesen Asmaa Mohammed ShamsEldeen
author_sort	Hend Ashour
title	Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
title_short	Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
title_full	Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
title_fullStr	Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
title_full_unstemmed	Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis
title_sort	vitamin d supplementation improves uterine receptivity in a rat model of vitamin d deficiency: a possible role of hoxa-10/fkbp52 axis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/08d2a0c0e5cf4f2185cbc5b3e5188ea2
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Vitamin D Supplementation Improves Uterine Receptivity in a Rat Model of Vitamin D Deficiency: A Possible Role of HOXA-10/FKBP52 Axis

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