Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil

Marina M Monobe,1 João P Araujo Junior,2 Kari V Lunsford,3 Rodrigo C Silva,4 Camilo Bulla41Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, 2Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University (UNESP), Botucatu,...

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Autores principales:	Monobe MM, Araujo Junior JP, Lunsford KV, Silva RC, Bulla C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/08d36be1915c45ed9e2329aaa21cf65c
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spelling	oai:doaj.org-article:08d36be1915c45ed9e2329aaa21cf65c2021-12-02T01:34:51ZFrequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil2230-2034https://doaj.org/article/08d36be1915c45ed9e2329aaa21cf65c2015-04-01T00:00:00Zhttp://www.dovepress.com/frequency-of-the-mdr1-mutant-allele-associated-with-multidrug-sensitiv-peer-reviewed-article-VMRRhttps://doaj.org/toc/2230-2034 Marina M Monobe,1 João P Araujo Junior,2 Kari V Lunsford,3 Rodrigo C Silva,4 Camilo Bulla41Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, 2Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University (UNESP), Botucatu, Brazil; 3Department of Clinical Sciences and Animal Health Center, 4Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi, MS, USAAbstract: To date, a 4-bp deletion in the MDR1 gene has been detected in more than ten dog breeds, as well as in mixed breed dogs, in several countries, however information regarding this mutation in dogs from Brazil is lacking. For this reason, 103 Collies, 77 Border Collies, 76 Shetland Sheepdogs, 20 Old English Sheepdogs, 55 German Shepherds, 16 Australian Shepherds, and 53 Whippets from Brazil were screened for the presence of the mutation. The heterozygous mutated genotype, MDR1 (+/−), frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 50.5% (95% CI =41.1%–59.9%), 31.3% (95% CI =8.6%–53.2%), and 15.8% (95% CI =7.7%–23.9%), respectively. Homozygous mutated genotype, MDR1 (−/−), was detected only in Collies 35.9%. The MDR1 allele mutant frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 61.2% (95% CI =54.8%–67.5%), 15.6% (95% CI =3.1%–28.2%), and 7.9% (95% CI =3.7%–12.1%), respectively. Additionally, even free of the mutant allele, the maximum mutant prevalence (MMP) in that population, with 95% CI, was 3.8%, 5.2%, 5.4%, and 13.8% for Border Collies, German Shepherds, Whippets, and Old English Sheepdogs, respectively. In this way, this information is important, not only for MDR1 genotype-based breeding programs and international exchange of breeding animals of predisposed breeds, but also for modification of drug therapy for breeds at risk.Keywords: P-glycoprotein, MDR1 mutation, ivermectin, dog, drug sensitivityMonobe MMAraujo Junior JPLunsford KVSilva RCBulla CDove Medical PressarticleVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol 2015, Iss default, Pp 111-117 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Veterinary medicine SF600-1100
spellingShingle	Veterinary medicine SF600-1100 Monobe MM Araujo Junior JP Lunsford KV Silva RC Bulla C Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
description	Marina M Monobe,1 João P Araujo Junior,2 Kari V Lunsford,3 Rodrigo C Silva,4 Camilo Bulla41Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, 2Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University (UNESP), Botucatu, Brazil; 3Department of Clinical Sciences and Animal Health Center, 4Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi, MS, USAAbstract: To date, a 4-bp deletion in the MDR1 gene has been detected in more than ten dog breeds, as well as in mixed breed dogs, in several countries, however information regarding this mutation in dogs from Brazil is lacking. For this reason, 103 Collies, 77 Border Collies, 76 Shetland Sheepdogs, 20 Old English Sheepdogs, 55 German Shepherds, 16 Australian Shepherds, and 53 Whippets from Brazil were screened for the presence of the mutation. The heterozygous mutated genotype, MDR1 (+/−), frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 50.5% (95% CI =41.1%–59.9%), 31.3% (95% CI =8.6%–53.2%), and 15.8% (95% CI =7.7%–23.9%), respectively. Homozygous mutated genotype, MDR1 (−/−), was detected only in Collies 35.9%. The MDR1 allele mutant frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 61.2% (95% CI =54.8%–67.5%), 15.6% (95% CI =3.1%–28.2%), and 7.9% (95% CI =3.7%–12.1%), respectively. Additionally, even free of the mutant allele, the maximum mutant prevalence (MMP) in that population, with 95% CI, was 3.8%, 5.2%, 5.4%, and 13.8% for Border Collies, German Shepherds, Whippets, and Old English Sheepdogs, respectively. In this way, this information is important, not only for MDR1 genotype-based breeding programs and international exchange of breeding animals of predisposed breeds, but also for modification of drug therapy for breeds at risk.Keywords: P-glycoprotein, MDR1 mutation, ivermectin, dog, drug sensitivity
format	article
author	Monobe MM Araujo Junior JP Lunsford KV Silva RC Bulla C
author_facet	Monobe MM Araujo Junior JP Lunsford KV Silva RC Bulla C
author_sort	Monobe MM
title	Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
title_short	Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
title_full	Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
title_fullStr	Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
title_full_unstemmed	Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil
title_sort	frequency of the mdr1 mutant allele associated with multidrug sensitivity in dogs from brazil
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/08d36be1915c45ed9e2329aaa21cf65c
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Frequency of the MDR1 mutant allele associated with multidrug sensitivity in dogs from Brazil

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