Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatograph...

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Autores principales:	Liqiang Liu, Liguang Xu, Steven Suryoprabowo, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunochromatographic assay monoclonal antibody mycotoxin ochratoxin a strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/08dd8552966646e2a6ff40bf78ea0e02
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spelling	oai:doaj.org-article:08dd8552966646e2a6ff40bf78ea0e022021-11-26T11:19:46ZDevelopment of an immunochromatographic test strip for the detection of ochratoxin A in red wine0954-01051465-344310.1080/09540105.2017.1401043https://doaj.org/article/08dd8552966646e2a6ff40bf78ea0e022018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1401043https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.Liqiang LiuLiguang XuSteven SuryoprabowoShanshan SongHua KuangTaylor & Francis Grouparticleimmunochromatographic assaymonoclonal antibodymycotoxinochratoxin astrip testAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 434-444 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunochromatographic assay monoclonal antibody mycotoxin ochratoxin a strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunochromatographic assay monoclonal antibody mycotoxin ochratoxin a strip test Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
description	Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.
format	article
author	Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang
author_facet	Liqiang Liu Liguang Xu Steven Suryoprabowo Shanshan Song Hua Kuang
author_sort	Liqiang Liu
title	Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
title_short	Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
title_full	Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
title_fullStr	Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
title_full_unstemmed	Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine
title_sort	development of an immunochromatographic test strip for the detection of ochratoxin a in red wine
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/08dd8552966646e2a6ff40bf78ea0e02
work_keys_str_mv	AT liqiangliu developmentofanimmunochromatographicteststripforthedetectionofochratoxinainredwine AT liguangxu developmentofanimmunochromatographicteststripforthedetectionofochratoxinainredwine AT stevensuryoprabowo developmentofanimmunochromatographicteststripforthedetectionofochratoxinainredwine AT shanshansong developmentofanimmunochromatographicteststripforthedetectionofochratoxinainredwine AT huakuang developmentofanimmunochromatographicteststripforthedetectionofochratoxinainredwine
_version_	1718409516597379072

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

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