High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica

Summary The natural plant product bisabolene serves as a precursor for the production of a wide range of industrially relevant chemicals. However, the low abundance of bisabolene in plants renders its isolation from plant sources non‐economically viable. Therefore, creation of microbial cell factori...

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Autores principales:	Yakun Zhao, Kun Zhu, Jian Li, Yu Zhao, Shenglong Li, Cuiying Zhang, Dongguang Xiao, Aiqun Yu
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/08de2b1b736145a2a6e11e14a63af7fe
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spelling	oai:doaj.org-article:08de2b1b736145a2a6e11e14a63af7fe2021-11-18T15:39:52ZHigh‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica1751-791510.1111/1751-7915.13768https://doaj.org/article/08de2b1b736145a2a6e11e14a63af7fe2021-11-01T00:00:00Zhttps://doi.org/10.1111/1751-7915.13768https://doaj.org/toc/1751-7915Summary The natural plant product bisabolene serves as a precursor for the production of a wide range of industrially relevant chemicals. However, the low abundance of bisabolene in plants renders its isolation from plant sources non‐economically viable. Therefore, creation of microbial cell factories for bisabolene production supported by synthetic biology and metabolic engineering strategies presents a more competitive and environmentally sustainable method for industrial production of bisabolene. In this proof‐of‐principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce α‐bisabolene, β‐bisabolene and γ‐bisabolene through heterologous expression of the α‐bisabolene synthase from Abies grandis, the β‐bisabolene synthase gene from Zingiber officinale and the γ‐bisabolene synthase gene from Helianthus annuus respectively. Subsequently, two metabolic engineering approaches, including overexpression of the endogenous mevalonate pathway genes and introduction of heterologous multidrug efflux transporters, were employed in order to improve bisabolene production. Furthermore, the fermentation conditions were optimized to maximize bisabolene production by the engineered Y. lipolytica strains from glucose. Finally, we explored the potential of the engineered Y. lipolytica strains for bisabolene production from the waste cooking oil. To our knowledge, this is the first report of bisabolene production in Y. lipolytica using metabolic engineering strategies. These findings provide valuable insights into the engineering of Y. lipolytica for a higher‐level production of bisabolene and its utilization in converting waste cooking oil into various industrially valuable products.Yakun ZhaoKun ZhuJian LiYu ZhaoShenglong LiCuiying ZhangDongguang XiaoAiqun YuWileyarticleBiotechnologyTP248.13-248.65ENMicrobial Biotechnology, Vol 14, Iss 6, Pp 2497-2513 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65
spellingShingle	Biotechnology TP248.13-248.65 Yakun Zhao Kun Zhu Jian Li Yu Zhao Shenglong Li Cuiying Zhang Dongguang Xiao Aiqun Yu High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
description	Summary The natural plant product bisabolene serves as a precursor for the production of a wide range of industrially relevant chemicals. However, the low abundance of bisabolene in plants renders its isolation from plant sources non‐economically viable. Therefore, creation of microbial cell factories for bisabolene production supported by synthetic biology and metabolic engineering strategies presents a more competitive and environmentally sustainable method for industrial production of bisabolene. In this proof‐of‐principle study, for the first time, we engineered the oleaginous yeast Yarrowia lipolytica to produce α‐bisabolene, β‐bisabolene and γ‐bisabolene through heterologous expression of the α‐bisabolene synthase from Abies grandis, the β‐bisabolene synthase gene from Zingiber officinale and the γ‐bisabolene synthase gene from Helianthus annuus respectively. Subsequently, two metabolic engineering approaches, including overexpression of the endogenous mevalonate pathway genes and introduction of heterologous multidrug efflux transporters, were employed in order to improve bisabolene production. Furthermore, the fermentation conditions were optimized to maximize bisabolene production by the engineered Y. lipolytica strains from glucose. Finally, we explored the potential of the engineered Y. lipolytica strains for bisabolene production from the waste cooking oil. To our knowledge, this is the first report of bisabolene production in Y. lipolytica using metabolic engineering strategies. These findings provide valuable insights into the engineering of Y. lipolytica for a higher‐level production of bisabolene and its utilization in converting waste cooking oil into various industrially valuable products.
format	article
author	Yakun Zhao Kun Zhu Jian Li Yu Zhao Shenglong Li Cuiying Zhang Dongguang Xiao Aiqun Yu
author_facet	Yakun Zhao Kun Zhu Jian Li Yu Zhao Shenglong Li Cuiying Zhang Dongguang Xiao Aiqun Yu
author_sort	Yakun Zhao
title	High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
title_short	High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
title_full	High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
title_fullStr	High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
title_full_unstemmed	High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica
title_sort	high‐efficiency production of bisabolene from waste cooking oil by metabolically engineered yarrowia lipolytica
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/08de2b1b736145a2a6e11e14a63af7fe
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High‐efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica

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