Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization

Although many undergraduate and graduate Cell and Molecular Biology courses study the bacterial cell cycle and the mechanisms that regulate prokaryotic cell division, few laboratory projects exist for the enhanced study of cell cycle characteristics in a standard teaching laboratory. One notable rea...

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Autores principales:	Lin Lin, Abha Choudhary, Anish Bavishi, Norma Ogbonna, Sarah Maddux, Madhusudan Choudhary
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2012
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/08e3ff3c31474a69afd2cdfb7aff4c74
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spelling	oai:doaj.org-article:08e3ff3c31474a69afd2cdfb7aff4c742021-11-15T15:20:09ZUse of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization10.1128/jmbe.v13i1.3461935-78851935-7877https://doaj.org/article/08e3ff3c31474a69afd2cdfb7aff4c742012-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v13i1.346https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Although many undergraduate and graduate Cell and Molecular Biology courses study the bacterial cell cycle and the mechanisms that regulate prokaryotic cell division, few laboratory projects exist for the enhanced study of cell cycle characteristics in a standard teaching laboratory. One notable reason for this lack of engaging laboratory projects is, although bacterial cells can be grown fairly easily, these cultured cells are in a variety of cell cycle states. As such, to study and understand the factors that regulate bacterial cell division in morphological, physiological, and even molecular respects, it is necessary to have bacterial cells in the same stage of its cell cycle. This matching can be performed by a procedure called cell cycle synchronization.Lin LinAbha ChoudharyAnish BavishiNorma OgbonnaSarah MadduxMadhusudan ChoudharyAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 13, Iss 1, Pp 50-53 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Lin Lin Abha Choudhary Anish Bavishi Norma Ogbonna Sarah Maddux Madhusudan Choudhary Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
description	Although many undergraduate and graduate Cell and Molecular Biology courses study the bacterial cell cycle and the mechanisms that regulate prokaryotic cell division, few laboratory projects exist for the enhanced study of cell cycle characteristics in a standard teaching laboratory. One notable reason for this lack of engaging laboratory projects is, although bacterial cells can be grown fairly easily, these cultured cells are in a variety of cell cycle states. As such, to study and understand the factors that regulate bacterial cell division in morphological, physiological, and even molecular respects, it is necessary to have bacterial cells in the same stage of its cell cycle. This matching can be performed by a procedure called cell cycle synchronization.
format	article
author	Lin Lin Abha Choudhary Anish Bavishi Norma Ogbonna Sarah Maddux Madhusudan Choudhary
author_facet	Lin Lin Abha Choudhary Anish Bavishi Norma Ogbonna Sarah Maddux Madhusudan Choudhary
author_sort	Lin Lin
title	Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
title_short	Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
title_full	Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
title_fullStr	Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
title_full_unstemmed	Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization
title_sort	use of the sucrose gradient method for bacterial cell cycle synchronization
publisher	American Society for Microbiology
publishDate	2012
url	https://doaj.org/article/08e3ff3c31474a69afd2cdfb7aff4c74
work_keys_str_mv	AT linlin useofthesucrosegradientmethodforbacterialcellcyclesynchronization AT abhachoudhary useofthesucrosegradientmethodforbacterialcellcyclesynchronization AT anishbavishi useofthesucrosegradientmethodforbacterialcellcyclesynchronization AT normaogbonna useofthesucrosegradientmethodforbacterialcellcyclesynchronization AT sarahmaddux useofthesucrosegradientmethodforbacterialcellcyclesynchronization AT madhusudanchoudhary useofthesucrosegradientmethodforbacterialcellcyclesynchronization
_version_	1718428110666334208

Use of the Sucrose Gradient Method for Bacterial Cell Cycle Synchronization

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