Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy

Systemic pilocarpine treatment is one of the most reliable means of inducing temporal lobe epilepsy (TLE). However, the traditional pilocarpine injection protocol using mice was associated with a high death rate, possibly because of cardiorespiratory collapse following status epilepticus (SE). To pr...

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Autores principales:	Alexandre Vigier, Nicolas Partouche, François J. Michel, Valérie Crépel, Thomas Marissal
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pilocarpine Caffeine Epilepsy Mice Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/08ebf41de9724880a1f0d534eab0d80b
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spelling	oai:doaj.org-article:08ebf41de9724880a1f0d534eab0d80b2021-12-04T04:33:11ZSubstantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy1095-953X10.1016/j.nbd.2021.105547https://doaj.org/article/08ebf41de9724880a1f0d534eab0d80b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0969996121002965https://doaj.org/toc/1095-953XSystemic pilocarpine treatment is one of the most reliable means of inducing temporal lobe epilepsy (TLE). However, the traditional pilocarpine injection protocol using mice was associated with a high death rate, possibly because of cardiorespiratory collapse following status epilepticus (SE). To prevent this, we developed a modified procedure of pilocarpine SE induction, which included a single injection of a moderate dose of caffeine during the induction phase. That new protocol was based on the use of young male mice as well as on a refined Racine's scale. Using that protocol, we report a substantially increased survival rate, thus enabling the generation of a large cohort of mice that exhibited cardinal histological (e.g., mossy fiber sprouting) and electrophysiological (e.g., chronic interictal events and ictal seizures) characteristics associated with TLE. In conclusion, our refined caffeine- and pilocarpine-based protocol substantially improves the outcome of the reliable pilocarpine mouse model of TLE.Alexandre VigierNicolas PartoucheFrançois J. MichelValérie CrépelThomas MarissalElsevierarticlePilocarpineCaffeineEpilepsyMiceNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENNeurobiology of Disease, Vol 161, Iss , Pp 105547- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pilocarpine Caffeine Epilepsy Mice Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	Pilocarpine Caffeine Epilepsy Mice Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Alexandre Vigier Nicolas Partouche François J. Michel Valérie Crépel Thomas Marissal Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
description	Systemic pilocarpine treatment is one of the most reliable means of inducing temporal lobe epilepsy (TLE). However, the traditional pilocarpine injection protocol using mice was associated with a high death rate, possibly because of cardiorespiratory collapse following status epilepticus (SE). To prevent this, we developed a modified procedure of pilocarpine SE induction, which included a single injection of a moderate dose of caffeine during the induction phase. That new protocol was based on the use of young male mice as well as on a refined Racine's scale. Using that protocol, we report a substantially increased survival rate, thus enabling the generation of a large cohort of mice that exhibited cardinal histological (e.g., mossy fiber sprouting) and electrophysiological (e.g., chronic interictal events and ictal seizures) characteristics associated with TLE. In conclusion, our refined caffeine- and pilocarpine-based protocol substantially improves the outcome of the reliable pilocarpine mouse model of TLE.
format	article
author	Alexandre Vigier Nicolas Partouche François J. Michel Valérie Crépel Thomas Marissal
author_facet	Alexandre Vigier Nicolas Partouche François J. Michel Valérie Crépel Thomas Marissal
author_sort	Alexandre Vigier
title	Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
title_short	Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
title_full	Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
title_fullStr	Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
title_full_unstemmed	Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
title_sort	substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/08ebf41de9724880a1f0d534eab0d80b
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Substantial outcome improvement using a refined pilocarpine mouse model of temporal lobe epilepsy

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