The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis

Abstract Light is an important environmental factor that regulates various physiological processes of fungi. To thoroughly study the responses of Monascus to blue light, transcriptome sequencing was performed on mRNAs isolated from samples of Monascus purpureus M9 cultured under three conditions: da...

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Autores principales:	Di Chen, Mianhua Chen, Shufen Wu, Zhenjing Li, Hua Yang, Changlu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08f3eeb74f394594a2bc7af3fd4bfe4a
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spelling	oai:doaj.org-article:08f3eeb74f394594a2bc7af3fd4bfe4a2021-12-02T11:52:23ZThe molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis10.1038/s41598-017-05990-x2045-2322https://doaj.org/article/08f3eeb74f394594a2bc7af3fd4bfe4a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05990-xhttps://doaj.org/toc/2045-2322Abstract Light is an important environmental factor that regulates various physiological processes of fungi. To thoroughly study the responses of Monascus to blue light, transcriptome sequencing was performed on mRNAs isolated from samples of Monascus purpureus M9 cultured under three conditions: darkness (D); exposure to blue light for 15 min/d (B15); and exposure to blue light for 60 min/d over 8 days (B60). The number of differentially expressed genes between the three pairs of samples—B15 vs D, B60 vs B15, and B60 vs D—was 1167, 1172, and 220, respectively. KEGG analysis showed the genes involved in primary metabolism including carbon and nitrogen metabolism were downregulated by B15 light treatment, whereas B15 upregulated expression of genes involved with aromatic amino acid metabolism, which associated with development, and branched chain amino acid metabolism, and fatty acid degradation, which can produce the biosynthetic precursors of pigments. When exposed to B60 conditions, genes with roles in carbohydrate metabolism and protein synthesis were upregulated as part of a stress response to blue light. Based on this study, we propose a predicted light-stimulated signal transduction pathway in Monascus. Our work is the first comprehensive investigation concerning the mechanism of Monascus responses to blue light.Di ChenMianhua ChenShufen WuZhenjing LiHua YangChanglu WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Di Chen Mianhua Chen Shufen Wu Zhenjing Li Hua Yang Changlu Wang The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
description	Abstract Light is an important environmental factor that regulates various physiological processes of fungi. To thoroughly study the responses of Monascus to blue light, transcriptome sequencing was performed on mRNAs isolated from samples of Monascus purpureus M9 cultured under three conditions: darkness (D); exposure to blue light for 15 min/d (B15); and exposure to blue light for 60 min/d over 8 days (B60). The number of differentially expressed genes between the three pairs of samples—B15 vs D, B60 vs B15, and B60 vs D—was 1167, 1172, and 220, respectively. KEGG analysis showed the genes involved in primary metabolism including carbon and nitrogen metabolism were downregulated by B15 light treatment, whereas B15 upregulated expression of genes involved with aromatic amino acid metabolism, which associated with development, and branched chain amino acid metabolism, and fatty acid degradation, which can produce the biosynthetic precursors of pigments. When exposed to B60 conditions, genes with roles in carbohydrate metabolism and protein synthesis were upregulated as part of a stress response to blue light. Based on this study, we propose a predicted light-stimulated signal transduction pathway in Monascus. Our work is the first comprehensive investigation concerning the mechanism of Monascus responses to blue light.
format	article
author	Di Chen Mianhua Chen Shufen Wu Zhenjing Li Hua Yang Changlu Wang
author_facet	Di Chen Mianhua Chen Shufen Wu Zhenjing Li Hua Yang Changlu Wang
author_sort	Di Chen
title	The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
title_short	The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
title_full	The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
title_fullStr	The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
title_full_unstemmed	The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis
title_sort	molecular mechanisms of monascus purpureus m9 responses to blue light based on the transcriptome analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/08f3eeb74f394594a2bc7af3fd4bfe4a
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The molecular mechanisms of Monascus purpureus M9 responses to blue light based on the transcriptome analysis

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