Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is...

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Autores principales:	Secil Kivrak Kiran, Selin Galatali, Sevil Yeniocak, Damla Ekin Ozkaya, Taner Mercan, Sevinc Guldag, Onur Celik, Naeem Abdul Ghafoor, Eergun Kaya
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	Charcoal Fe-EDDHA hazelnut ISSR micropropagation Biology (General) QH301-705.5 Botany QK1-989
Acceso en línea:	https://doaj.org/article/08f4114867294728869692c56e8ed54c
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spelling	oai:doaj.org-article:08f4114867294728869692c56e8ed54c2021-11-08T13:15:10ZInvestigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.10.36253/ahsc-105360394-61691592-1573https://doaj.org/article/08f4114867294728869692c56e8ed54c2021-08-01T00:00:00Zhttps://oaj.fupress.net/index.php/ahs/article/view/10536https://doaj.org/toc/0394-6169https://doaj.org/toc/1592-1573 Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%. Secil Kivrak KiranSelin GalataliSevil YeniocakDamla Ekin OzkayaTaner MercanSevinc GuldagOnur CelikNaeem Abdul GhafoorEergun KayaFirenze University PressarticleCharcoalFe-EDDHAhazelnutISSRmicropropagationBiology (General)QH301-705.5BotanyQK1-989ENAdvances in Horticultural Science, Vol 35, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Charcoal Fe-EDDHA hazelnut ISSR micropropagation Biology (General) QH301-705.5 Botany QK1-989
spellingShingle	Charcoal Fe-EDDHA hazelnut ISSR micropropagation Biology (General) QH301-705.5 Botany QK1-989 Secil Kivrak Kiran Selin Galatali Sevil Yeniocak Damla Ekin Ozkaya Taner Mercan Sevinc Guldag Onur Celik Naeem Abdul Ghafoor Eergun Kaya Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
description	Cultivation of Corylus avellana L. in Turkey is performed generally in the northern regions where it is an important source of livelihood for the local farmers. More than 70% of world hazelnut production is supplied by Turkey, but compared with other countries, Turkey’s hazelnut production area is quite narrow. In this study was aimed to develop an effective in vitro production for seven local cultivars of C. avellana. Therefore, WPM medium supplemented with 6-Benzylaminopurine (BAP) was modified by using single or in combination of Fe-EDDHA, AgNO3, H3BO3, charcoal and gibberellic acid. In all varieties, the best regeneration rates varying between 68% and 94% were obtained from WPM medium supplemented with 4.4 µM BAP, 27.8 µM Fe-EDDHA and 10g/L Charcoal. Genetic stability of shoots derived from meristem culture using the best medium was analysed using ISSR primers, when the gel images of the PCR products were examined, no polymorphic band was observed in samples collected from seven provinces, and the genetic stability was determined as 100%.
format	article
author	Secil Kivrak Kiran Selin Galatali Sevil Yeniocak Damla Ekin Ozkaya Taner Mercan Sevinc Guldag Onur Celik Naeem Abdul Ghafoor Eergun Kaya
author_facet	Secil Kivrak Kiran Selin Galatali Sevil Yeniocak Damla Ekin Ozkaya Taner Mercan Sevinc Guldag Onur Celik Naeem Abdul Ghafoor Eergun Kaya
author_sort	Secil Kivrak Kiran
title	Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
title_short	Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
title_full	Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
title_fullStr	Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
title_full_unstemmed	Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.
title_sort	investigation of modified wpm medium for the best meristem proliferation of corylus avellana l.
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/08f4114867294728869692c56e8ed54c
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Investigation of modified WPM medium for the best meristem proliferation of Corylus avellana L.

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