In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.

Breast cancer cells with the CD44+/CD24- phenotype have been reported to be tumourigenic due to their enhanced capacity for cancer development and their self-renewal potential. The identification of human tumourigenic breast cancer cells in surgical samples has recently received increased attention...

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Autores principales:	Giuseppe Perrone, Laura Maria Gaeta, Mariagiovanna Zagami, Francesca Nasorri, Roberto Coppola, Domenico Borzomati, Francesco Bartolozzi, Vittorio Altomare, Lucio Trodella, Giuseppe Tonini, Daniele Santini, Andrea Cavani, Andrea Onetti Muda
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/08fa3383c40f4ab1b45540c582703303
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spelling	oai:doaj.org-article:08fa3383c40f4ab1b45540c5827033032021-11-18T07:05:45ZIn situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.1932-620310.1371/journal.pone.0043110https://doaj.org/article/08fa3383c40f4ab1b45540c5827033032012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028444/?tool=EBIhttps://doaj.org/toc/1932-6203Breast cancer cells with the CD44+/CD24- phenotype have been reported to be tumourigenic due to their enhanced capacity for cancer development and their self-renewal potential. The identification of human tumourigenic breast cancer cells in surgical samples has recently received increased attention due to the implications for prognosis and treatment, although limitations exist in the interpretation of these studies. To better identify the CD44+/CD24- cells in routine surgical specimens, 56 primary breast carcinoma cases were analysed by immunofluorescence and confocal microscopy, and the results were compared using flow cytometry analysis to correlate the amount and distribution of the CD44+/CD24- population with clinicopathological features. Using these methods, we showed that the breast carcinoma cells displayed four distinct sub-populations based on the expression pattern of CD44 and CD24. The CD44+/CD24- cells were found in 91% of breast tumours and constituted an average of 6.12% (range, 0.11%-21.23%) of the tumour. A strong correlation was found between the percentage of CD44+/CD24- cells in primary tumours and distant metastasis development (p = 0.0001); in addition, there was an inverse significant association with ER and PGR status (p = 0.002 and p = 0.001, respectively). No relationship was evident with tumour size (T) and regional lymph node (N) status, differentiation grade, proliferative index or HER2 status. In a multivariate analysis, the percentage of CD44+/CD24- cancer cells was an independent factor related to metastasis development (p = 0.004). Our results indicate that confocal analysis of fluorescence-labelled breast cancer samples obtained at surgery is a reliable method to identify the CD44+/CD24- tumourigenic cell population, allowing for the stratification of breast cancer patients into two groups with substantially different relapse rates on the basis of CD44+/CD24- cell percentage.Giuseppe PerroneLaura Maria GaetaMariagiovanna ZagamiFrancesca NasorriRoberto CoppolaDomenico BorzomatiFrancesco BartolozziVittorio AltomareLucio TrodellaGiuseppe ToniniDaniele SantiniAndrea CavaniAndrea Onetti MudaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e43110 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giuseppe Perrone Laura Maria Gaeta Mariagiovanna Zagami Francesca Nasorri Roberto Coppola Domenico Borzomati Francesco Bartolozzi Vittorio Altomare Lucio Trodella Giuseppe Tonini Daniele Santini Andrea Cavani Andrea Onetti Muda In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
description	Breast cancer cells with the CD44+/CD24- phenotype have been reported to be tumourigenic due to their enhanced capacity for cancer development and their self-renewal potential. The identification of human tumourigenic breast cancer cells in surgical samples has recently received increased attention due to the implications for prognosis and treatment, although limitations exist in the interpretation of these studies. To better identify the CD44+/CD24- cells in routine surgical specimens, 56 primary breast carcinoma cases were analysed by immunofluorescence and confocal microscopy, and the results were compared using flow cytometry analysis to correlate the amount and distribution of the CD44+/CD24- population with clinicopathological features. Using these methods, we showed that the breast carcinoma cells displayed four distinct sub-populations based on the expression pattern of CD44 and CD24. The CD44+/CD24- cells were found in 91% of breast tumours and constituted an average of 6.12% (range, 0.11%-21.23%) of the tumour. A strong correlation was found between the percentage of CD44+/CD24- cells in primary tumours and distant metastasis development (p = 0.0001); in addition, there was an inverse significant association with ER and PGR status (p = 0.002 and p = 0.001, respectively). No relationship was evident with tumour size (T) and regional lymph node (N) status, differentiation grade, proliferative index or HER2 status. In a multivariate analysis, the percentage of CD44+/CD24- cancer cells was an independent factor related to metastasis development (p = 0.004). Our results indicate that confocal analysis of fluorescence-labelled breast cancer samples obtained at surgery is a reliable method to identify the CD44+/CD24- tumourigenic cell population, allowing for the stratification of breast cancer patients into two groups with substantially different relapse rates on the basis of CD44+/CD24- cell percentage.
format	article
author	Giuseppe Perrone Laura Maria Gaeta Mariagiovanna Zagami Francesca Nasorri Roberto Coppola Domenico Borzomati Francesco Bartolozzi Vittorio Altomare Lucio Trodella Giuseppe Tonini Daniele Santini Andrea Cavani Andrea Onetti Muda
author_facet	Giuseppe Perrone Laura Maria Gaeta Mariagiovanna Zagami Francesca Nasorri Roberto Coppola Domenico Borzomati Francesco Bartolozzi Vittorio Altomare Lucio Trodella Giuseppe Tonini Daniele Santini Andrea Cavani Andrea Onetti Muda
author_sort	Giuseppe Perrone
title	In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
title_short	In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
title_full	In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
title_fullStr	In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
title_full_unstemmed	In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.
title_sort	in situ identification of cd44+/cd24- cancer cells in primary human breast carcinomas.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/08fa3383c40f4ab1b45540c582703303
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In situ identification of CD44+/CD24- cancer cells in primary human breast carcinomas.

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