Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran

Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: th...

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Autores principales:	Mahtab Hamidpour, Saman Mahdavi
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	bacillus cereus raw milk ces gene cytk gene Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/09050b25808a4eceac78f372e486a8e9
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spelling	oai:doaj.org-article:09050b25808a4eceac78f372e486a8e92021-11-18T06:56:46ZPrevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran2345-33622322-586610.34172/ijep.2020.17https://doaj.org/article/09050b25808a4eceac78f372e486a8e92020-08-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4311https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.Mahtab HamidpourSaman MahdaviAlborz University of Medical Sciencesarticlebacillus cereus raw milk ces gene cytk gene Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 3, Pp 76-79 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus cereus raw milk ces gene cytk gene Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	bacillus cereus raw milk ces gene cytk gene Diseases of the digestive system. Gastroenterology RC799-869 Mahtab Hamidpour Saman Mahdavi Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
description	Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.
format	article
author	Mahtab Hamidpour Saman Mahdavi
author_facet	Mahtab Hamidpour Saman Mahdavi
author_sort	Mahtab Hamidpour
title	Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
title_short	Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
title_full	Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
title_fullStr	Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
title_full_unstemmed	Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
title_sort	prevalence of ces and cytk genes of bacillus cereus isolated from raw milk in tabriz, iran
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/09050b25808a4eceac78f372e486a8e9
work_keys_str_mv	AT mahtabhamidpour prevalenceofcesandcytkgenesofbacilluscereusisolatedfromrawmilkintabriziran AT samanmahdavi prevalenceofcesandcytkgenesofbacilluscereusisolatedfromrawmilkintabriziran
_version_	1718424147850166272

Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran

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