Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application

Vinod Vellora Thekkae Padil, Miroslav ČerníkLaboratory of Chemical Remediation Processes, Institute for Nanomaterials, Advanced Technology and Innovation, Technical University of Liberec, Studentská 1402/2, Liberec, Czech RepublicBackground: Copper oxide (CuO) nanoparti...

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Autores principales: Thekkae Padil VV, Černík M
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2013
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/0906ad4353df41518c8e0a0af64d884e
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spelling oai:doaj.org-article:0906ad4353df41518c8e0a0af64d884e2021-12-02T02:42:33ZGreen synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application1176-91141178-2013https://doaj.org/article/0906ad4353df41518c8e0a0af64d884e2013-02-01T00:00:00Zhttp://www.dovepress.com/green-synthesis-of-copper-oxide-nanoparticles-using-gum-karaya-as-a-bi-a12326https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Vinod Vellora Thekkae Padil, Miroslav ČerníkLaboratory of Chemical Remediation Processes, Institute for Nanomaterials, Advanced Technology and Innovation, Technical University of Liberec, Studentská 1402/2, Liberec, Czech RepublicBackground: Copper oxide (CuO) nanoparticles have attracted huge attention due to catalytic, electric, optical, photonic, textile, nanofluid, and antibacterial activity depending on the size, shape, and neighboring medium. In the present paper, we synthesized CuO nanoparticles using gum karaya, a natural nontoxic hydrocolloid, by green technology and explored its potential antibacterial application.Methods: The CuO nanoparticles were synthesized by a colloid-thermal synthesis process. The mixture contained various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and 3 mM) and gum karaya (10 mg/mL) and was kept at 75°C at 250 rpm for 1 hour in an orbital shaker. The synthesized CuO was purified and dried to obtain different sizes of the CuO nanoparticles. The well diffusion method was used to study the antibacterial activity of the synthesized CuO nanoparticles. The zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration were determined by the broth microdilution method recommended by the Clinical and Laboratory Standards Institute.Results: Scanning electron microscopy analysis showed CuO nanoparticles evenly distributed on the surface of the gum matrix. X-ray diffraction of the synthesized nanoparticles indicates the formation of single-phase CuO with a monoclinic structure. The Fourier transform infrared spectroscopy peak at 525 cm−1 should be a stretching of CuO, which matches up to the B2u mode. The peaks at 525 cm−1 and 580 cm−1 indicated the formation of CuO nanostructure. Transmission electron microscope analyses revealed CuO nanoparticles of 4.8 ± 1.6 nm, 5.5 ± 2.5 nm, and 7.8 ± 2.3 nm sizes were synthesized with various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and 3 mM). X-ray photoelectron spectroscopy profiles indicated that the O 1s and Cu 2p peak corresponding to the CuO nanoparticles were observed. The antibacterial activity of the synthesized nanoparticles was tested against Gram-negative and positive cultures.Conclusion: The formed CuO nanoparticles are small in size (4.8 ± 1.6 nm), highly stable, and have significant antibacterial action on both the Gram classes of bacteria compared to larger sizes of synthesized CuO (7.8 ± 2.3 nm) nanoparticles. The smaller size of the CuO nanoparticles (4.8 ± 1.6 nm) was found to be yielding a maximum zone of inhibition compared to the larger size of synthesized CuO nanoparticles (7.8 ± 2.3 nm). The results also indicate that increase in precursor concentration enhances an increase in particle size, as well as the morphology of synthesized CuO nanoparticles.Keywords: gum karaya, CuO nanoparticles, XRD, FTIR, XPS, antibacterial activityThekkae Padil VVČerník MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 889-898 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Thekkae Padil VV
Černík M
Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
description Vinod Vellora Thekkae Padil, Miroslav ČerníkLaboratory of Chemical Remediation Processes, Institute for Nanomaterials, Advanced Technology and Innovation, Technical University of Liberec, Studentská 1402/2, Liberec, Czech RepublicBackground: Copper oxide (CuO) nanoparticles have attracted huge attention due to catalytic, electric, optical, photonic, textile, nanofluid, and antibacterial activity depending on the size, shape, and neighboring medium. In the present paper, we synthesized CuO nanoparticles using gum karaya, a natural nontoxic hydrocolloid, by green technology and explored its potential antibacterial application.Methods: The CuO nanoparticles were synthesized by a colloid-thermal synthesis process. The mixture contained various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and 3 mM) and gum karaya (10 mg/mL) and was kept at 75°C at 250 rpm for 1 hour in an orbital shaker. The synthesized CuO was purified and dried to obtain different sizes of the CuO nanoparticles. The well diffusion method was used to study the antibacterial activity of the synthesized CuO nanoparticles. The zone of inhibition, minimum inhibitory concentration, and minimum bactericidal concentration were determined by the broth microdilution method recommended by the Clinical and Laboratory Standards Institute.Results: Scanning electron microscopy analysis showed CuO nanoparticles evenly distributed on the surface of the gum matrix. X-ray diffraction of the synthesized nanoparticles indicates the formation of single-phase CuO with a monoclinic structure. The Fourier transform infrared spectroscopy peak at 525 cm−1 should be a stretching of CuO, which matches up to the B2u mode. The peaks at 525 cm−1 and 580 cm−1 indicated the formation of CuO nanostructure. Transmission electron microscope analyses revealed CuO nanoparticles of 4.8 ± 1.6 nm, 5.5 ± 2.5 nm, and 7.8 ± 2.3 nm sizes were synthesized with various concentrations of CuCl2 · 2H2O (1 mM, 2 mM, and 3 mM). X-ray photoelectron spectroscopy profiles indicated that the O 1s and Cu 2p peak corresponding to the CuO nanoparticles were observed. The antibacterial activity of the synthesized nanoparticles was tested against Gram-negative and positive cultures.Conclusion: The formed CuO nanoparticles are small in size (4.8 ± 1.6 nm), highly stable, and have significant antibacterial action on both the Gram classes of bacteria compared to larger sizes of synthesized CuO (7.8 ± 2.3 nm) nanoparticles. The smaller size of the CuO nanoparticles (4.8 ± 1.6 nm) was found to be yielding a maximum zone of inhibition compared to the larger size of synthesized CuO nanoparticles (7.8 ± 2.3 nm). The results also indicate that increase in precursor concentration enhances an increase in particle size, as well as the morphology of synthesized CuO nanoparticles.Keywords: gum karaya, CuO nanoparticles, XRD, FTIR, XPS, antibacterial activity
format article
author Thekkae Padil VV
Černík M
author_facet Thekkae Padil VV
Černík M
author_sort Thekkae Padil VV
title Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
title_short Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
title_full Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
title_fullStr Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
title_full_unstemmed Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
title_sort green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application
publisher Dove Medical Press
publishDate 2013
url https://doaj.org/article/0906ad4353df41518c8e0a0af64d884e
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