Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

Abstract The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, wh...

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Autores principales:	Sinead Connolly, David Newport, Kieran McGourty
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/09094a5bb7bf47e2af732f8c4d9c1764
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spelling	oai:doaj.org-article:09094a5bb7bf47e2af732f8c4d9c17642021-12-02T15:39:41ZCell specific variation in viability in suspension in in vitro Poiseuille flow conditions10.1038/s41598-021-91865-12045-2322https://doaj.org/article/09094a5bb7bf47e2af732f8c4d9c17642021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91865-1https://doaj.org/toc/2045-2322Abstract The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T-cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes.Sinead ConnollyDavid NewportKieran McGourtyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sinead Connolly David Newport Kieran McGourty Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
description	Abstract The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T-cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes.
format	article
author	Sinead Connolly David Newport Kieran McGourty
author_facet	Sinead Connolly David Newport Kieran McGourty
author_sort	Sinead Connolly
title	Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_short	Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_full	Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_fullStr	Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_full_unstemmed	Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_sort	cell specific variation in viability in suspension in in vitro poiseuille flow conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/09094a5bb7bf47e2af732f8c4d9c1764
work_keys_str_mv	AT sineadconnolly cellspecificvariationinviabilityinsuspensionininvitropoiseuilleflowconditions AT davidnewport cellspecificvariationinviabilityinsuspensionininvitropoiseuilleflowconditions AT kieranmcgourty cellspecificvariationinviabilityinsuspensionininvitropoiseuilleflowconditions
_version_	1718385852224110592

Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

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