The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro
Zinc plays a very important role in various biological activities of the body. Multifaceted role of zinc is also known in testes development, spermatogenesis, capacitation and has effect on spermatozoa motility. On the other hand, the growing industry of nanotechnology has created reasonable interes...
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Elsevier
2021
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oai:doaj.org-article:090dc6d4223b4b6fa9ffe9f72ebf5e3c2021-11-20T04:57:07ZThe effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro1319-562X10.1016/j.sjbs.2021.08.045https://doaj.org/article/090dc6d4223b4b6fa9ffe9f72ebf5e3c2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21007348https://doaj.org/toc/1319-562XZinc plays a very important role in various biological activities of the body. Multifaceted role of zinc is also known in testes development, spermatogenesis, capacitation and has effect on spermatozoa motility. On the other hand, the growing industry of nanotechnology has created reasonable interest of the risk assessment for nanoparticles. The aim of this study was to evaluate in vitro effect of zinc oxide (ZnO) nanoparticles on rabbit spermatozoa. Fresh semen was collected from sexually mature New Zealand rabbits. Experimental groups were prepared by diluting semen with ZnO nanoparticles in seven different concentrations (6–391 mg/mL). The experimental groups were compared with control group. Semen was assessed using computer assisted semen analysis (CASA) at intervals of 0, 1, 2 and 3 h of incubation. The mitochondrial toxicity assay (MTT) assay was used to determine cell viability. The results of monitored motility parameters in experimental groups showed a decreasing trend during whole experiment. Significant decrease (P < 0.001) of motility and progressive motility was observed after 3 h of incubation in samples cultured with higher ZnO nanoparticles in comparison to the control group. After 3 h of incubation, viability of rabbit spermatozoa showed slightly increased values in group with the lowest concentration of ZnO nanoparticles, but in other groups viability showed non-significant decrease compared to control. Similar tendency was detected for spermatozoa membrane integrity. These original data show the negative dose–dependent effect of ZnO nanoparticles on spermatozoa motility and viability parameters.Marko Halo JrKlaudia BułkaPiotr A. AntosAgnieszka GreńTomáš SlaninaĽubomír OndruškaKatarína TokárováMartin MassányiGrzegorz FormickiMarko HaloPeter MassányiElsevierarticleZnO nanoparticlesSpermatozoaMotilityViabilityRabbitBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7450-7454 (2021) |
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EN |
| topic |
ZnO nanoparticles Spermatozoa Motility Viability Rabbit Biology (General) QH301-705.5 |
| spellingShingle |
ZnO nanoparticles Spermatozoa Motility Viability Rabbit Biology (General) QH301-705.5 Marko Halo Jr Klaudia Bułka Piotr A. Antos Agnieszka Greń Tomáš Slanina Ľubomír Ondruška Katarína Tokárová Martin Massányi Grzegorz Formicki Marko Halo Peter Massányi The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| description |
Zinc plays a very important role in various biological activities of the body. Multifaceted role of zinc is also known in testes development, spermatogenesis, capacitation and has effect on spermatozoa motility. On the other hand, the growing industry of nanotechnology has created reasonable interest of the risk assessment for nanoparticles. The aim of this study was to evaluate in vitro effect of zinc oxide (ZnO) nanoparticles on rabbit spermatozoa. Fresh semen was collected from sexually mature New Zealand rabbits. Experimental groups were prepared by diluting semen with ZnO nanoparticles in seven different concentrations (6–391 mg/mL). The experimental groups were compared with control group. Semen was assessed using computer assisted semen analysis (CASA) at intervals of 0, 1, 2 and 3 h of incubation. The mitochondrial toxicity assay (MTT) assay was used to determine cell viability. The results of monitored motility parameters in experimental groups showed a decreasing trend during whole experiment. Significant decrease (P < 0.001) of motility and progressive motility was observed after 3 h of incubation in samples cultured with higher ZnO nanoparticles in comparison to the control group. After 3 h of incubation, viability of rabbit spermatozoa showed slightly increased values in group with the lowest concentration of ZnO nanoparticles, but in other groups viability showed non-significant decrease compared to control. Similar tendency was detected for spermatozoa membrane integrity. These original data show the negative dose–dependent effect of ZnO nanoparticles on spermatozoa motility and viability parameters. |
| format |
article |
| author |
Marko Halo Jr Klaudia Bułka Piotr A. Antos Agnieszka Greń Tomáš Slanina Ľubomír Ondruška Katarína Tokárová Martin Massányi Grzegorz Formicki Marko Halo Peter Massányi |
| author_facet |
Marko Halo Jr Klaudia Bułka Piotr A. Antos Agnieszka Greń Tomáš Slanina Ľubomír Ondruška Katarína Tokárová Martin Massányi Grzegorz Formicki Marko Halo Peter Massányi |
| author_sort |
Marko Halo Jr |
| title |
The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| title_short |
The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| title_full |
The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| title_fullStr |
The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| title_full_unstemmed |
The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| title_sort |
effect of zno nanoparticles on rabbit spermatozoa motility and viability parameters in vitro |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/090dc6d4223b4b6fa9ffe9f72ebf5e3c |
| work_keys_str_mv |
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