Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges

Atefe Rezaei,1,2 Jaleh Varshosaz,3 Mehrafarin Fesharaki,4 Armin Farhang,2 Seid Mahdi Jafari51Department of Food Science and Technology, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran; 2Food Security Research Center, Isfahan University of Medical Sciences,...

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Autores principales:	Rezaei A, Varshosaz J, Fesharaki M, Farhang A, Jafari SM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Nanoencapsulation nanosponges cyclodextrins ferulic acid cytotoxicity. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/09123ce440074f3fa46843ce29aceedd
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spelling	oai:doaj.org-article:09123ce440074f3fa46843ce29aceedd2021-12-02T04:18:21ZImproving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges1178-2013https://doaj.org/article/09123ce440074f3fa46843ce29aceedd2019-06-01T00:00:00Zhttps://www.dovepress.com/improving-the-solubility-and-in-vitro-cytotoxicity-anticancer-activity-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Atefe Rezaei,1,2 Jaleh Varshosaz,3 Mehrafarin Fesharaki,4 Armin Farhang,2 Seid Mahdi Jafari51Department of Food Science and Technology, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran; 2Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; 3Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran; 4Department of Cell Science, Research Center Medical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; 5Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranPurpose: Ferulic acid (FA) is a poorly water-soluble natural antioxidant with anticancer activity. This poor solubility limits the application of FA in the food and pharmaceutical industry. Cyclodextrin nanosponges (CD-NSs) are a novel group of cross-linked CD derivatives which can be used to enhance the solubility of low-soluble bioactive compounds.Methods: In this study, FA was encapsulated into the NSs in the proportion of 1:4 (FA:NS). Diphenyl carbonate was used as a cross-linker in different proportions with β-CD. Characterization of obtained NSs was performed using scanning electron microscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) analysis.Results: Our results revealed that the solubility of encapsulated FA was increased up to fifteenfold compared with pure FA in the proportion of 1:4 (CD:cross-linker). The results of FTIR, XRD, and DSC confirmed the interaction of FA with NSs. The cytotoxicity of encapsulated FA against MCF7 and 4T1 breast cancer cell lines was investigated using different concentrations of FA in 24, 48, and 72 hrs. The cytotoxicity assay indicated that FA treatment reduced viability and enhanced apoptosis of cancer cells. IC50 value of encapsulated FA (250 ppm) was decreased by threefold when compared with pure FA (750 ppm).Conclusion: In general, CD-NS was found to be a suitable delivery system for poorly soluble bioactives such as FA.Keywords: nanoencapsulation, nanosponges, cyclodextrins, ferulic acid, cytotoxicityRezaei AVarshosaz JFesharaki MFarhang AJafari SMDove Medical PressarticleNanoencapsulationnanospongescyclodextrinsferulic acidcytotoxicity.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4589-4599 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoencapsulation nanosponges cyclodextrins ferulic acid cytotoxicity. Medicine (General) R5-920
spellingShingle	Nanoencapsulation nanosponges cyclodextrins ferulic acid cytotoxicity. Medicine (General) R5-920 Rezaei A Varshosaz J Fesharaki M Farhang A Jafari SM Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
description	Atefe Rezaei,1,2 Jaleh Varshosaz,3 Mehrafarin Fesharaki,4 Armin Farhang,2 Seid Mahdi Jafari51Department of Food Science and Technology, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran; 2Food Security Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; 3Department of Pharmaceutics, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran; 4Department of Cell Science, Research Center Medical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; 5Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranPurpose: Ferulic acid (FA) is a poorly water-soluble natural antioxidant with anticancer activity. This poor solubility limits the application of FA in the food and pharmaceutical industry. Cyclodextrin nanosponges (CD-NSs) are a novel group of cross-linked CD derivatives which can be used to enhance the solubility of low-soluble bioactive compounds.Methods: In this study, FA was encapsulated into the NSs in the proportion of 1:4 (FA:NS). Diphenyl carbonate was used as a cross-linker in different proportions with β-CD. Characterization of obtained NSs was performed using scanning electron microscopy, X-ray diffraction (XRD), differential scanning calorimetry (DSC), and Fourier transform infrared (FTIR) analysis.Results: Our results revealed that the solubility of encapsulated FA was increased up to fifteenfold compared with pure FA in the proportion of 1:4 (CD:cross-linker). The results of FTIR, XRD, and DSC confirmed the interaction of FA with NSs. The cytotoxicity of encapsulated FA against MCF7 and 4T1 breast cancer cell lines was investigated using different concentrations of FA in 24, 48, and 72 hrs. The cytotoxicity assay indicated that FA treatment reduced viability and enhanced apoptosis of cancer cells. IC50 value of encapsulated FA (250 ppm) was decreased by threefold when compared with pure FA (750 ppm).Conclusion: In general, CD-NS was found to be a suitable delivery system for poorly soluble bioactives such as FA.Keywords: nanoencapsulation, nanosponges, cyclodextrins, ferulic acid, cytotoxicity
format	article
author	Rezaei A Varshosaz J Fesharaki M Farhang A Jafari SM
author_facet	Rezaei A Varshosaz J Fesharaki M Farhang A Jafari SM
author_sort	Rezaei A
title	Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
title_short	Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
title_full	Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
title_fullStr	Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
title_full_unstemmed	Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
title_sort	improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/09123ce440074f3fa46843ce29aceedd
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Improving the solubility and in vitro cytotoxicity (anticancer activity) of ferulic acid by loading it into cyclodextrin nanosponges

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