Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism

ABSTRACT As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeosta...

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Autores principales:	Casey M. Gries, Marat R. Sadykov, Logan L. Bulock, Sujata S. Chaudhari, Vinai C. Thomas, Jeffrey L. Bose, Kenneth W. Bayles
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Staphylococcus aureus metabolism potassium transport Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/09162e47ad4c4c13a544086d18260f25
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spelling	oai:doaj.org-article:09162e47ad4c4c13a544086d18260f252021-11-15T15:21:18ZPotassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism10.1128/mSphere.00125-162379-5042https://doaj.org/article/09162e47ad4c4c13a544086d18260f252016-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00125-16https://doaj.org/toc/2379-5042ABSTRACT As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K+) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K+ uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K+ deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K+ uptake in maintaining essential components of S. aureus metabolism. IMPORTANCE Previous studies describing mechanisms for K+ uptake in S. aureus revealed that the Ktr-mediated K+ transport system was required for normal growth under alkaline conditions but not under neutral or acidic conditions. This work focuses on the effect of K+ uptake on S. aureus metabolism, including intracellular pH and carbon flux, and is the first to utilize a pH-dependent green fluorescent protein (GFP) to measure S. aureus cytoplasmic pH. These studies highlight the role of K+ uptake in supporting proton efflux under alkaline conditions and uncover a critical role for K+ uptake in establishing efficient carbon utilization.Casey M. GriesMarat R. SadykovLogan L. BulockSujata S. ChaudhariVinai C. ThomasJeffrey L. BoseKenneth W. BaylesAmerican Society for MicrobiologyarticleStaphylococcus aureusmetabolismpotassium transportMicrobiologyQR1-502ENmSphere, Vol 1, Iss 3 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus aureus metabolism potassium transport Microbiology QR1-502
spellingShingle	Staphylococcus aureus metabolism potassium transport Microbiology QR1-502 Casey M. Gries Marat R. Sadykov Logan L. Bulock Sujata S. Chaudhari Vinai C. Thomas Jeffrey L. Bose Kenneth W. Bayles Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
description	ABSTRACT As a leading cause of community-associated and nosocomial infections, Staphylococcus aureus requires sophisticated mechanisms that function to maintain cellular homeostasis in response to its exposure to changing environmental conditions. The adaptation to stress and maintenance of homeostasis depend largely on membrane activity, including supporting electrochemical gradients and synthesis of ATP. This is largely achieved through potassium (K+) transport, which plays an essential role in maintaining chemiosmotic homeostasis, affects antimicrobial resistance, and contributes to fitness in vivo. Here, we report that S. aureus Ktr-mediated K+ uptake is necessary for maintaining cytoplasmic pH and the establishment of a proton motive force. Metabolite analyses revealed that K+ deficiency affects both metabolic and energy states of S. aureus by impairing oxidative phosphorylation and directing carbon flux toward substrate-level phosphorylation. Taken together, these results underline the importance of K+ uptake in maintaining essential components of S. aureus metabolism. IMPORTANCE Previous studies describing mechanisms for K+ uptake in S. aureus revealed that the Ktr-mediated K+ transport system was required for normal growth under alkaline conditions but not under neutral or acidic conditions. This work focuses on the effect of K+ uptake on S. aureus metabolism, including intracellular pH and carbon flux, and is the first to utilize a pH-dependent green fluorescent protein (GFP) to measure S. aureus cytoplasmic pH. These studies highlight the role of K+ uptake in supporting proton efflux under alkaline conditions and uncover a critical role for K+ uptake in establishing efficient carbon utilization.
format	article
author	Casey M. Gries Marat R. Sadykov Logan L. Bulock Sujata S. Chaudhari Vinai C. Thomas Jeffrey L. Bose Kenneth W. Bayles
author_facet	Casey M. Gries Marat R. Sadykov Logan L. Bulock Sujata S. Chaudhari Vinai C. Thomas Jeffrey L. Bose Kenneth W. Bayles
author_sort	Casey M. Gries
title	Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
title_short	Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
title_full	Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
title_fullStr	Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
title_full_unstemmed	Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism
title_sort	potassium uptake modulates <named-content content-type="genus-species">staphylococcus aureus</named-content> metabolism
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/09162e47ad4c4c13a544086d18260f25
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Potassium Uptake Modulates <named-content content-type="genus-species">Staphylococcus aureus</named-content> Metabolism

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